Zhang Gong, Zhang Xiaoyan, Hu Yuanqing, Jiao Xin-An, Huang Jinlin
Jiangsu Key Lab of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 88 South Daxue Road, Yangzhou, 225009, Jiangsu, China.
Curr Microbiol. 2015 Sep;71(3):341-6. doi: 10.1007/s00284-015-0853-3. Epub 2015 Jun 23.
Campylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. In our study, we applied multilocus sequence typing (MLST) technology to 167 C. jejuni isolates from diverse sources in Eastern China to examine their genetic diversity. MLST defined 94 sequence types (STs) belonging to 18 clonal complexes (CCs). Forty-five STs from 60 isolates (36%) and 22 alleles have not been previously documented in an international database. One hundred and two isolates, accounting for 61.1% of all isolates, belonged to eight clonal complexes. The eight major CCs were also the most common complexes from different sources. The most common ST type of isolates from human and food was ST-353. The dominant ST type in chicken and foods was ST-354. Among 21 STs that contained two or more different sources isolates, 15 STs contained human isolates and isolates from other sources, suggesting that potentially pathogenic strains are not restricted to specific lineages.
空肠弯曲菌是一种主要的食源性病原体，在许多发达国家会引发人类肠胃炎。在我们的研究中，我们对来自中国东部不同来源的167株空肠弯曲菌分离株应用多位点序列分型（MLST）技术，以检测它们的遗传多样性。MLST确定了属于18个克隆复合体（CCs）的94种序列类型（STs）。60株分离株（36%）中的45种STs和22个等位基因此前未在国际数据库中记录过。102株分离株，占所有分离株的61.1%，属于8个克隆复合体。这8个主要的CCs也是来自不同来源的最常见复合体。来自人类和食品的分离株中最常见的ST类型是ST-353。鸡肉和食品中占主导地位的ST类型是ST-354。在包含两种或更多不同来源分离株的21种STs中，15种STs包含人类分离株和来自其他来源的分离株，这表明潜在致病菌株并不局限于特定谱系。
