Department of Oriental Medicine, Dongguk University, Kyung Ju 780-714, Republic of Korea.
Oncol Rep. 2012 May;27(5):1467-74. doi: 10.3892/or.2012.1637. Epub 2012 Jan 16.
Lycii cortex radicis (LCR) is a traditional Korean medicinal herb. The present study was undertaken to examine the effect of an LCR extract on glioma cell growth and to determine its molecular mechanism in U87MG human glioma cells. The LCR extract resulted in apoptotic cell death in a dose- and time-dependent manner. The LCR extract-induced cell death was associated with generation of reactive oxygen species (ROS). Western blot analysis showed that the LCR extract caused downregulation of Akt and ERK. The LCR-induced cell death was prevented by transfection with the constitutively active forms of Akt and MEK. Oral administration of LCR extracts in subcutaneous U87MG xenograft models reduced glioma tumor volume. Taken together, these findings suggest that the LCR extract results in human glioma cell death through mechanisms involving ROS generation, downregulation of Akt and ERK, and caspase activation in vitro and reduces glioma tumor growth in vivo. These data suggest that the LCR extract may serve as a potential therapeutic agent for malignant human glioblastomas.
枸杞根皮（LCR）是一种传统的韩国药草。本研究旨在探讨 LCR 提取物对神经胶质瘤细胞生长的影响，并确定其在 U87MG 人神经胶质瘤细胞中的分子机制。LCR 提取物以剂量和时间依赖的方式导致细胞凋亡。LCR 提取物诱导的细胞死亡与活性氧（ROS）的产生有关。Western blot 分析表明，LCR 提取物导致 Akt 和 ERK 的下调。用组成性激活形式的 Akt 和 MEK 转染可防止 LCR 诱导的细胞死亡。LCR 提取物在皮下 U87MG 异种移植模型中的口服给药可减少神经胶质瘤肿瘤体积。综上所述，这些发现表明，LCR 提取物通过涉及 ROS 生成、Akt 和 ERK 下调以及 caspase 激活的机制导致人神经胶质瘤细胞死亡，并在体内减少神经胶质瘤肿瘤生长。这些数据表明，LCR 提取物可能作为恶性人类神经胶质母细胞瘤的潜在治疗剂。
