Lustigman S, Huima T, Brotman B, Miller K, Prince A M
Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021.
Exp Parasitol. 1990 Nov;71(4):489-95. doi: 10.1016/0014-4894(90)90075-n.
The annulated cuticles of third- and fourth-stage larvae of Onchocerca volvulus have the typical structure of other nematodes but the cuticle of fourth-stage larvae was thinner. The surface of the third-stage larva was wrinkled and fuzzy, while that of the fourth-stage was smooth. Intermediate stages in the formation of the new cuticle and epicuticle beneath the old basal layer and of the separation of the cuticles are shown. Monoclonal antibodies specific to the surface of third-stage larvae did not react with the surface of the fourth-stage larvae. Binding of the monoclonal antibodies to the third-stage larvae was abrogated by treatment of the worms with trypsin and proteinase K, but was unaffected by treatment with periodate or the detergents sodium deoxycholate and SDS. The lectins RCA120 and WGA, but not any of the other lectins tested, bound only to the surface of fourth-stage larvae, and not to that of third-stage larvae. The surfaces of third- and fourth-stage larvae were shown to be different and contained stage-specific surface epitopes.
旋盘尾丝虫第三期和第四期幼虫的环纹角质层具有其他线虫的典型结构，但第四期幼虫的角质层较薄。第三期幼虫的表面有皱纹且模糊，而第四期幼虫的表面则很光滑。展示了在旧基底层下方新角质层和表皮的形成以及角质层分离的中间阶段。针对第三期幼虫表面的单克隆抗体与第四期幼虫的表面不发生反应。用胰蛋白酶和蛋白酶K处理蠕虫后，单克隆抗体与第三期幼虫的结合被消除，但用高碘酸盐或去污剂脱氧胆酸钠和十二烷基硫酸钠处理则不影响这种结合。凝集素RCA120和WGA，但不是所测试的其他任何凝集素，仅与第四期幼虫的表面结合，而不与第三期幼虫的表面结合。第三期和第四期幼虫的表面显示不同，且含有阶段特异性的表面表位。
