由于隐性 ucpA 基因，大肠杆菌对呋喃的耐受性增加。
Increased furan tolerance in Escherichia coli due to a cryptic ucpA gene.
机构信息
Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA.
出版信息
Appl Environ Microbiol. 2012 Apr;78(7):2452-5. doi: 10.1128/AEM.07783-11. Epub 2012 Jan 20.
Abstract
Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.
摘要
表达谱芯片被用于鉴定出 4 种推测的氧化还原酶，它们受糠醛（15mM，15 分钟）诱导表达上调（>3 倍）。一个（ucpA）的质粒表达增加了工程菌 LY180 和野生型菌 W 对呋喃的耐受性。ucpA 的缺失降低了糠醛的耐受性。尽管其机制尚不清楚，但隐性的 ucpA 基因现在与表型相关：呋喃抗性。
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