SlyA 与 Dickeya dadantii 3937 中的 T3SS 主调控因子 HrpL 平行调节 III 型分泌系统（T3SS）基因。
SlyA regulates type III secretion system (T3SS) genes in parallel with the T3SS master regulator HrpL in Dickeya dadantii 3937.
机构信息
Department of Biological Sciences, University of Wisconsin, Milwaukee, Wisconsin, USA.
出版信息
Appl Environ Microbiol. 2012 Apr;78(8):2888-95. doi: 10.1128/AEM.07021-11. Epub 2012 Jan 20.
Abstract
The hypersensitive response and pathogenicity (hrp) genes of Dickeya dadantii 3937 encode a type III secretion system (T3SS) which is essential for its full virulence. Previous studies of the T3SS regulation in D. dadantii 3937 revealed that the expression of the hrp genes is regulated by a master regulator, HrpL, through the HrpX-HrpY-HrpS-HrpL and GacS-GacA-rsmB-RsmA pathways. In this work, we identified a novel regulator of the SlyA/MarR family, SlyA, which regulates hrp genes of the HrpL regulon in parallel with HrpL in D. dadantii. SlyA regulates the T3SS in a two-tier manner. It negatively regulates the expression of hrpL by downregulating hrpS and upregulating rsmA. Interestingly, concomitant with its downregulation of the hrpL, SlyA positively regulates the expression of hrpA and hrpN, two hrp genes located in the HrpL regulon. In contrast to Pectobacterium carotovorum, the expression of slyA is not controlled by ExpR and ExpI in D. dadantii 3937. We further show that SlyA is involved in controlling swimming motility and pellicle formation in D. dadantii 3937.
摘要
戴克氏菌 3937 的超敏反应和致病性 (hrp) 基因编码一种 III 型分泌系统 (T3SS)，这对于其完全毒力是必不可少的。先前对戴克氏菌 3937 中 T3SS 调控的研究表明，hrp 基因的表达受主调控因子 HrpL 通过 HrpX-HrpY-HrpS-HrpL 和 GacS-GacA-rsmB-RsmA 途径调控。在这项工作中，我们鉴定了一种 SlyA/MarR 家族的新型调控因子 SlyA，它与 HrpL 一起在戴克氏菌中平行调控 HrpL 调控子中的 hrp 基因。SlyA 通过下调 hrpS 和上调 rsmA 以两级方式调控 T3SS。有趣的是，SlyA 下调 hrpL 的同时，还正向调控位于 HrpL 调控子中的两个 hrp 基因 hrpA 和 hrpN 的表达。与果胶杆菌不同，在戴克氏菌 3937 中 slyA 的表达不受 ExpR 和 ExpI 的调控。我们进一步表明，SlyA 参与控制戴克氏菌 3937 的游泳运动和菌膜形成。
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J Bacteriol. 2011 Apr;193(8):2076-7. doi: 10.1128/JB.01513-10. Epub 2011 Jan 7.
2
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3
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4
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Mol Microbiol. 2010 Aug;77(3):787-800. doi: 10.1111/j.1365-2958.2010.07246.x. Epub 2010 Jun 23.
5
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J Bacteriol. 2009 Sep;191(17):5409-18. doi: 10.1128/JB.00240-09. Epub 2009 Jun 19.
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7
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