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鱿鱼视网膜中视黄醛色素(视网膜光异构酶)cDNA的克隆及核苷酸序列分析

Cloning and nucleotide sequence of cDNA for retinochrome, retinal photoisomerase from the squid retina.

作者信息

Hara-Nishimura I, Matsumoto T, Mori H, Nishimura M, Hara R, Hara T

机构信息

Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.

出版信息

FEBS Lett. 1990 Oct 1;271(1-2):106-10. doi: 10.1016/0014-5793(90)80383-t.

DOI:10.1016/0014-5793(90)80383-t
PMID:2226795
Abstract

The Rhodopsin-retinochrome system is essential for the visual photoreception of molluscs. cDNA coding for retinochrome of the squid (Todarodes pacificus) was cloned and the nucleotide sequence has been determined. The sequence (2.1 kb) covers the whole coding region of 903 bp. The deduced primary sequence suggests that retinochrome contains seven transmembrane spanning domains. The homology with bovine rhodopsin and the possible retinal binding site are also discussed.

摘要

视紫红质-视黄醛色素系统对于软体动物的视觉光感受至关重要。克隆了编码鱿鱼(太平洋褶柔鱼)视黄醛色素的cDNA,并确定了其核苷酸序列。该序列(2.1 kb)覆盖了903 bp的整个编码区域。推导的一级序列表明视黄醛色素含有七个跨膜结构域。还讨论了与牛视紫红质的同源性以及可能的视黄醛结合位点。

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Cloning and nucleotide sequence of cDNA for retinochrome, retinal photoisomerase from the squid retina.鱿鱼视网膜中视黄醛色素(视网膜光异构酶)cDNA的克隆及核苷酸序列分析
FEBS Lett. 1990 Oct 1;271(1-2):106-10. doi: 10.1016/0014-5793(90)80383-t.
2
Cloning and nucleotide sequence of cDNA for rhodopsin of the squid Todarodes pacificus.太平洋褶柔鱼视紫红质cDNA的克隆及核苷酸序列
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Amino acid sequence surrounding the retinal-binding site in retinochrome of the squid, Todarodes pacificus.太平洋褶柔鱼视紫红质中视网膜结合位点周围的氨基酸序列。
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Highly conserved glutamic acid in the extracellular IV-V loop in rhodopsins acts as the counterion in retinochrome, a member of the rhodopsin family.视紫红质细胞外IV-V环中高度保守的谷氨酸在视网膜色素(视紫红质家族成员之一)中充当抗衡离子。
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Retinal-binding protein as a shuttle for retinal in the rhodopsin-retinochrome system of the squid visual cells.视网膜结合蛋白作为视网膜在鱿鱼视觉细胞视紫红质-视黄醛色素系统中传递视网膜的载体。
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