二维凝胶电泳显示的正常和少精子症患者人类精子之间的蛋白质差异
Protein differences between normal and oligospermic human sperm demonstrated by two-dimensional gel electrophoresis.
作者信息
Morgentaler A, Schopperle W M, Crocker R H, DeWolf W C
机构信息
Division of Urology, Beth Israel Hospital, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
Fertil Steril. 1990 Nov;54(5):902-5. doi: 10.1016/s0015-0282(16)53953-3.
Protein expression by sperm obtained from men with normal semen analysis and men with oligospermia were evaluated by two-dimensional gel electrophoresis. Proteins were solubilized in a 9.5 M urea/2% Nonidet-P40 (LKB, Bromma, Sweden) lysis buffer and underwent second dimension separation on 10 to 16% polyacrylamide gradient gels. A set of 36 invariant proteins was identified in all normospermic samples, whereas 8 of 10 evaluable oligospermic samples lacked 1 or more of the invariant proteins. Proteins absent in oligospermic samples may be critical to normal sperm function and may serve as markers for infertility.
通过二维凝胶电泳对精液分析正常男性和少精子症男性的精子进行蛋白质表达评估。蛋白质在9.5M尿素/2%非离子去污剂P40（瑞典布罗玛LKB公司）裂解缓冲液中溶解，并在10%至16%聚丙烯酰胺梯度凝胶上进行第二维分离。在所有正常精子样本中鉴定出一组36种不变蛋白，而10个可评估的少精子症样本中有8个缺乏1种或更多的不变蛋白。少精子症样本中缺失的蛋白质可能对正常精子功能至关重要，并可能作为不孕症的标志物。
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