Fogel M, Yamanishi K, Rapp F
Int J Cancer. 1979 May 15;23(5):657-62. doi: 10.1002/ijc.2910230511.
Enhancement of host cell reactivation (HCR) of ultraviolet (UV)-irradiated herpes simplex virus (HSV) was demonstrated in cell cultures pretreated with caffeine, hydroxyurea, or 5-bromodeoxyuridine (BrdUrd). The effect of caffeine on HCR was shown to depend on the time of drug treatment with respect to infection. In cultures treated with caffeine during the course of virus replication, the infectivity of irradiated HSV was reduced about nine-fold, while cultures pretreated with the drug before infection showed an increase in infectivity. The extent of HCR enhancement depended on the time interval between treatment with caffeine and infection, drug concentration, and the UV irradiation dose to which HSV was exposed. Magnitude of enhancement of HCR by caffeine differed in various cell species. The results suggest that enhanced HCR of UV-irradiated HSV by DNA antimetabolities is associated with DNA repair activated in consequence of cell DNA damage.
在经咖啡因、羟基脲或5-溴脱氧尿苷（BrdUrd）预处理的细胞培养物中，已证实紫外线（UV）照射的单纯疱疹病毒（HSV）的宿主细胞复活（HCR）增强。咖啡因对HCR的影响显示取决于药物处理相对于感染的时间。在病毒复制过程中用咖啡因处理的培养物中，照射后HSV的感染性降低了约9倍，而在感染前用该药物预处理的培养物中感染性增加。HCR增强的程度取决于咖啡因处理与感染之间的时间间隔、药物浓度以及HSV所暴露的紫外线照射剂量。咖啡因对HCR增强的幅度在不同细胞种类中有所不同。结果表明，DNA抗代谢物对紫外线照射的HSV的HCR增强与细胞DNA损伤导致激活的DNA修复有关。
