Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Appl Biochem Biotechnol. 2012 Jul;167(5):1254-69. doi: 10.1007/s12010-012-9547-5. Epub 2012 Jan 22.
Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the most favorable substrate than haritaki, pomegranate, tea leaf waste and arjun fruit. Physicochemical parameters were initially optimized using OVAT methodology and some important factors like incubation time, incubation temperature, substrate:moisture ratio as well as carbon, nitrogen and phosphate concentrations were verified with Box-Behken design of response surface methodology. Phosphate source, nitrogen source and temperature were found as the most favorable variables in the maximization of production. Tannase production was enhanced from 1.536 U/g to 5.784 U/g using tamarind seed OVAT optimization and further enhancement up to 6.15 U/g following RSM. An overall 3.76- and 4.0-fold increases in tannase production were achieved in OVAT and RSM, respectively.
采用“单因素法”（One Variable at a Time，OVAT）和响应面法（Response Surface Methodology，RSM）对新分离的青霉（Penicillium purpurogenum）PAF6 进行了单宁酶生产研究。利用富含单宁的植物残渣作为支持固体基质和唯一碳源，其中，罗望子种子比诃子、石榴、茶叶废料和余甘子更适合作为底物。采用 OVAT 方法对理化参数进行了初步优化，并采用 Box-Behken 设计的响应面法对一些重要因素（如培养时间、培养温度、底物与水分比以及碳、氮和磷酸盐浓度）进行了验证。发现磷酸盐源、氮源和温度是提高产量的最有利变量。采用罗望子种子 OVAT 优化使单宁酶产量从 1.536 U/g 提高到 5.784 U/g，随后通过 RSM 进一步提高到 6.15 U/g。在 OVAT 和 RSM 中，单宁酶的产量分别提高了 3.76 倍和 4.0 倍。
