Sabu A, Pandey A, Daud M Jaafar, Szakacs G
Biotechnology Division, Regional Research Laboratory, Council of Scientific and Industrial Research (CSIR), Trivandrum 695 019, India.
Bioresour Technol. 2005 Jul;96(11):1223-8. doi: 10.1016/j.biortech.2004.11.002. Epub 2005 Jan 1.
Palm kernel cake (PKC), the residue obtained after extraction of palm oil from oil palm seeds and tamarind seed powder (TSP) obtained after removing the fruit pulp from tamarind fruit pod were tested for the production of tannase under solid-state fermentation (SSF) using Aspergillus niger ATCC 16620. The fungal strain was grown on the substrates without any pretreatment. In PKC medium, a maximum enzyme yield of 13.03 IU/g dry substrate (gds) was obtained when SSF was carried out at 30 degrees C, 53.5% initial substrate moisture, 33 x 10(9) spores/5 g substrate inoculum size and 5% tannic acid as additional carbon source after 96 h of fermentation. In TSP medium, maximum tannase yield of 6.44 IU/gds was obtained at 30 degrees C, 65.75% initial substrate moisture, 11 x 10(9) spores/5 g substrate inoculum, 1% glycerol as additional carbon source and 1% potassium nitrate as additional nitrogen source after 120 h of fermentation. Results from the study are promising for the economic utilization and value addition of these important agro residues, which are abundantly available in many tropical and subtropical countries.
使用黑曲霉ATCC 16620，对从油棕种子中提取棕榈油后得到的棕榈仁饼（PKC）以及从罗望子果荚中去除果肉后得到的罗望子种子粉（TSP）进行固态发酵（SSF）生产单宁酶的测试。该真菌菌株在未经任何预处理的底物上生长。在PKC培养基中，当在30℃、初始底物水分含量53.5%、接种量为33×10⁹个孢子/5g底物且添加5%单宁酸作为额外碳源的条件下进行96小时固态发酵后，获得的最大酶产量为13.03 IU/g干底物（gds）。在TSP培养基中，当在30℃、初始底物水分含量65.75%、接种量为11×10⁹个孢子/5g底物、添加1%甘油作为额外碳源以及1%硝酸钾作为额外氮源的条件下进行120小时发酵后，获得的最大单宁酶产量为6.44 IU/gds。该研究结果对于这些重要农业残余物的经济利用和增值具有前景，这些残余物在许多热带和亚热带国家大量存在。
