Marks R
Department of Medicine (Dermatology), University of Wales College of Medicine, Cardiff, UK.
J Int Med Res. 1990;18 Suppl 3:29C-34C.
Quantitative techniques for assessing the effects of tretinoin on photo-aged skin mainly involve humans, although hairless mice were used in initial studies. Ideally techniques should be non-invasive but occasionally biopsies have to be taken, especially when studying the effects of tretinoin on different skin compartments. Characteristic features of photo-aged skin, including the development of fine and coarse wrinkles, skin discoloration, rosy cheeks and telangiectasis, have been assessed subjectively using a visual analogue scale. Effects of tretinoin on wrinkle depth have been measured non-invasively by quantifying contours of silicone rubber replicas using either a mechanical tracking device or an optical technique employing an image-analysing computer. Changes in skin colour have been measured using an erythema meter and the stimulatory effect of tretinoin on blood flow has been established by laser doppler flowmetry. Skin thickness has been measured non-invasively using pulsed A-scan ultrasound which showed that tropical tretinoin increased thickness. Biopsies have also been used but no changes in the thickness of the dermal repair zone have been noted in humans, in contrast to the situation in hairless mice. Epidermal dysplasia has been measured by a visual analogue scale or by objective image analysis.
评估维甲酸对光老化皮肤影响的定量技术主要涉及人类，尽管在最初的研究中使用了无毛小鼠。理想情况下，技术应该是非侵入性的，但有时必须进行活检，特别是在研究维甲酸对不同皮肤层的影响时。光老化皮肤的特征，包括细纹和粗纹的形成、皮肤变色、脸颊发红和毛细血管扩张，已使用视觉模拟量表进行主观评估。通过使用机械跟踪装置或采用图像分析计算机的光学技术对硅橡胶复制品的轮廓进行量化，非侵入性地测量了维甲酸对皱纹深度的影响。使用红斑仪测量了皮肤颜色的变化，并通过激光多普勒血流仪确定了维甲酸对血流的刺激作用。使用脉冲A扫描超声非侵入性地测量了皮肤厚度，结果表明外用维甲酸可增加皮肤厚度。也进行了活检，但与无毛小鼠的情况相反，在人类中未观察到真皮修复区厚度的变化。表皮发育异常已通过视觉模拟量表或客观图像分析进行测量。
