Benes H, Edmondson R G, Fink P, Kejzlarová-Lepesant J, Lepesant J A, Miles J P, Spivey D W
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205.
Dev Biol. 1990 Nov;142(1):138-46. doi: 10.1016/0012-1606(90)90157-e.
Previous work has demonstrated expression of the larval serum protein-2 gene, Lsp-2, uniquely during the late larval and pupal stages of development in Drosophila melanogaster. Here we report that the LSP-2 polypeptide accumulates in the hemolymph throughout adult life as well. Western blot analysis using an LSP-2 antiserum reveals notable differences in the molecular weight of the larval and adult polypeptides. Lsp-2 transcription results in a unique mRNA of 2.3 kb, exhibiting the same 5' end in both larvae and adults. However, adult Lsp-2 mRNa is only expressed at 1% of the high level detectable in late third-instar larvae. Whereas Lsp-2 mRNA accumulates uniformly in all fat body cells of third-instar larvae, over 80% of the adult Lsp-2 transcript is expressed in the adipose tissue of the head. These results suggest a differential regulation for expression of the Drosophila Lsp-2 gene in adults and larvae.
先前的研究表明，果蝇幼虫血清蛋白2基因（Lsp-2）仅在黑腹果蝇发育的幼虫晚期和蛹期表达。在此我们报告，LSP-2多肽在成虫整个生命周期中也会在血淋巴中积累。使用LSP-2抗血清进行的蛋白质印迹分析显示，幼虫和成虫多肽的分子量存在显著差异。Lsp-2转录产生一种独特的2.3 kb mRNA，在幼虫和成虫中具有相同的5'末端。然而，成虫Lsp-2 mRNA的表达水平仅为三龄晚期幼虫中可检测到的高水平的1%。虽然Lsp-2 mRNA在三龄幼虫的所有脂肪体细胞中均匀积累，但超过80%的成虫Lsp-2转录本在头部的脂肪组织中表达。这些结果表明，果蝇Lsp-2基因在成虫和幼虫中的表达存在差异调节。
