Morrissey R E, Fowler B A, Harris M W, Moorman M P, Jameson C W, Schwetz B A
Division of Toxicology Research and Testing, National Toxicology Program, NIEHS, Research Triangle Park, North Carolina 27711.
Fundam Appl Toxicol. 1990 Aug;15(2):350-6. doi: 10.1016/0272-0590(90)90060-w.
Arsine gas is a potent hemolytic agent but the effects of exposure to tolerated concentrations on pregnancy and prenatal development have not been reported. In the present evaluation, groups of bred mice and rats were exposed to arsine at concentrations of 0.025, 0.5, or 2.5 ppm on Gestation Days (gd) 6 through 15. Animals were killed on gd 17 (mice) or on gd 20 (rats) and endpoints of maternal and developmental toxicity were evaluated. In rats, maternal spleens were enlarged in the 2.5 ppm group and there was a decrease in packed red cell volume in pregnant rats. Fetuses weighed more than in the control group but other endpoints of developmental toxicity were not affected by arsine exposure. In another experiment involving separate groups of rats, the arsenic content of maternal blood and fetal livers increased with increasing atmospheric arsine concentrations, as assessed on gd 20. In mice, maternal spleen size was significantly increased in the 2.5 ppm group. The number of live fetuses, mean fetal body weight, and percentages of resorptions or malformations per litter were not affected by arsine exposure. In conclusion, arsine at atmospheric concentrations that caused increases in maternal spleen size and measurable levels of arsenic in maternal blood and fetal livers did not adversely affect endpoints of developmental toxicity.
砷化氢气体是一种强效溶血剂，但关于暴露于耐受浓度下对妊娠和产前发育的影响尚无报道。在本评估中，将妊娠第6至15天的繁殖小鼠和大鼠分组，分别暴露于浓度为0.025、0.5或2.5 ppm的砷化氢环境中。在妊娠第17天（小鼠）或第20天（大鼠）处死动物，并评估母体和发育毒性终点。在大鼠中，2.5 ppm组母体脾脏肿大，妊娠大鼠的红细胞压积降低。胎儿体重高于对照组，但砷化氢暴露对其他发育毒性终点无影响。在另一项涉及不同组大鼠的实验中，如在妊娠第20天评估的那样，母体血液和胎儿肝脏中的砷含量随大气中砷化氢浓度的增加而增加。在小鼠中，2.5 ppm组母体脾脏大小显著增加。活胎数量、平均胎儿体重以及每窝吸收或畸形的百分比不受砷化氢暴露的影响。总之，在大气浓度下，砷化氢虽导致母体脾脏大小增加以及母体血液和胎儿肝脏中砷含量可测，但并未对发育毒性终点产生不利影响。
