Sakuma Akiko, Nishiyama Shoko, Yasuno Kyohei, Ohmuro Tamio, Kamiie Junichi, Shirota Kinji
J Toxicol Pathol. 2010 Dec;23(4):265-9. doi: 10.1293/tox.23.265. Epub 2010 Dec 16.
Cutaneous clear cell adnexal carcinoma was found in the right lip of a 14-year-old male castrated Shih Tzu. Histologically, the tumor mostly consisted of neoplastic cells with clear or vacuolated cytoplasms and contained frequent tubular structures. Neoplastic cells showed coexpression of pan-cytokeratin (CK) and vimentin by double-labeled immunofluorescence staining. In addition, immunohistochemistry revealed that the tumor cells were positive for pan-CK (AE1/AE3, KL1, CAM 5.2), CK-7, CK-8, CK-14, CK-15, CK-18, vimentin and alpha-smooth muscle actin (SMA) with varied intensity and positivity. Among these marker proteins, SMA was positive in 75% of the tumor cells. On the other hand, CK-15, which is a specific marker of follicular stem cells, was expressed in less than 1% of the tumor cells. Based on these findings, the tumor showed diverse differentiation in apocrine sweat glands and the inner and outer root sheaths of hair follicles, indicating the follicular stem cell to be the origin of this tumor.
在一只14岁去势的雄性西施犬的右侧唇部发现了皮肤透明细胞附属器癌。组织学上，肿瘤主要由具有透明或空泡状细胞质的肿瘤细胞组成，并含有频繁出现的管状结构。通过双标记免疫荧光染色，肿瘤细胞显示出泛细胞角蛋白（CK）和波形蛋白的共表达。此外，免疫组织化学显示肿瘤细胞对泛CK（AE1/AE3、KL1、CAM 5.2）、CK-7、CK-8、CK-14、CK-15、CK-18、波形蛋白和α-平滑肌肌动蛋白（SMA）呈不同强度和阳性反应。在这些标记蛋白中，SMA在75%的肿瘤细胞中呈阳性。另一方面，作为毛囊干细胞特异性标记物的CK-15，在不到1%的肿瘤细胞中表达。基于这些发现，该肿瘤在顶泌汗腺以及毛囊的内、外根鞘中显示出不同程度的分化，表明毛囊干细胞是该肿瘤的起源。
