YASUNO Kyohei, NISHIYAMA Shoko, SUETSUGU Fumio, OGIHARA Kikumi, MADARAME Hiroo, SHIROTA Kinji
Research Institute of Biosciences, Azabu University, Kawasaki, Kanagawa, Japan.
J Vet Med Sci. 2009 Nov;71(11):1513-7. doi: 10.1292/jvms.001513.
A 5-year-old, male Bichon-Frise dog presented with a cutaneous mass in the basal region of the auricle. Histologically, the cutaneous neoplasm was comprised of lobules with solid cellular proliferation separated by thin fibrous septa. Neoplastic cells varied in size, with moderate to abundant amounts of PAS-positive cytoplasm, large nuclei and prominent nucleoli. Immunohistochemical examinations showed that tumor cells were positive for pan-cytokeratin (CK) (AE1/AE3 and CAM5.2), CK8 and CK18, but negative for pan-CK (KL1), CK7, CK14, CK16 and CK20. Double-labeled immunofluorescence testing indicated that neoplastic cells frequently co-expressed CK and vimentin, suggesting divergent differentiation of tumor cells. Based on these findings, the tumor was diagnosed as canine clear cell adnexal carcinoma.
一只5岁雄性比熊犬耳廓基部出现一个皮肤肿物。组织学检查显示，该皮肤肿瘤由小叶组成，小叶内为实性细胞增生，被薄纤维间隔分隔。肿瘤细胞大小不一，胞质PAS阳性，含量中等至丰富，细胞核大，核仁明显。免疫组化检查显示，肿瘤细胞对泛细胞角蛋白（CK）（AE1/AE3和CAM5.2）、CK8和CK18呈阳性，但对泛CK（KL1）、CK7、CK14、CK16和CK20呈阴性。双标免疫荧光检测表明，肿瘤细胞常同时表达CK和波形蛋白，提示肿瘤细胞存在不同分化。基于这些发现，该肿瘤被诊断为犬透明细胞附件癌。
