Foster H M, Whitehead R H
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Australia.
Gastroenterology. 1990 Dec;99(6):1710-4. doi: 10.1016/0016-5085(90)90477-i.
The role of epidermal growth factor in the proliferation of normal and premalignant colonocytes in vivo is not fully understood. In particular, the relative importance of its possible systemic and intraluminal routes of action has not been fully clarified. Rats with surgically defunctioned distal colorectums were used, and mini osmotic pumps were implanted to study the effects of intraluminal and IV administration of epidermal growth factor on colonocyte proliferation. Within 2 weeks of bypass, colonocyte proliferation in defunctioned colorectum has decreased to about one third the rate in normal colorectum or in colorectum proximal to the defunctioning colostomy. Intraluminal epidermal growth factor, infused from the time of operation did not reverse this hypoplasia, whereas IV epidermal growth factor maintained colonocyte proliferation at approximately the normal rate in bypassed colorectum. This model is suitable for testing other putative colonic mitogens for possible intraluminal and systemic effects.
表皮生长因子在体内正常和癌前结肠细胞增殖中的作用尚未完全明确。特别是，其可能的全身和腔内作用途径的相对重要性尚未完全阐明。使用手术使远端结肠直肠失去功能的大鼠，并植入微型渗透泵，以研究腔内和静脉注射表皮生长因子对结肠细胞增殖的影响。在旁路手术2周内，失去功能的结肠直肠中的结肠细胞增殖已降至正常结肠直肠或造瘘口近端结肠直肠中增殖速率的约三分之一。从手术时开始注入腔内的表皮生长因子并未逆转这种发育不全，而静脉注射表皮生长因子可使旁路结肠直肠中的结肠细胞增殖维持在大致正常的速率。该模型适用于测试其他假定的结肠有丝分裂原的腔内和全身作用。
