British Antarctic Survey, Natural Environment Research Council, Cambridge, UK.
FEMS Microbiol Ecol. 2012 May;80(2):452-68. doi: 10.1111/j.1574-6941.2012.01313.x. Epub 2012 Mar 19.
Spatial patchiness in marine surface bacterioplankton populations was investigated in the Southern Ocean, where the Antarctic Circumpolar Current meets the islands of the Scotia Arc and is subjected to terrestrial input, upwelling of nutrients and seasonal phytoplankton blooms. Total bacterioplankton population density, group-specific taxonomic distribution and six of eight dominant members of the bacterioplankton community were found to be consistent across 18 nearshore sites at eight locations around the Scotia Arc. Results from seven independent 16S rRNA gene clone libraries (1223 sequences in total) and fluorescent in situ hybridization suggested that microbial assemblages were predominantly homogeneous between Scotia Arc sites, where the Alphaproteobacteria, Gammaproteobacteria and the Cytophaga-Flavobacterium-Bacteroidetes cluster were the dominant bacterial groups. Of the 1223 useable sequences generated, 1087 (89%) shared ≥ 97% similarity with marine microorganisms and 331 (27%) matched published sequences previously detected in permanently cold Arctic and Antarctic marine environments. Taken together, results suggest that the dominant bacterioplankton groups are consistent between locations, but significant differences may be detected across the rare biodiversity.
本研究调查了南大洋海洋表生细菌浮游种群的空间斑块性，该海域的南极绕极流与斯科舍弧形列岛相遇，并受到陆地输入、营养物质上升流和季节性浮游植物繁殖的影响。在斯科舍弧形列岛周围八个地点的 18 个近岸地点，发现总细菌浮游种群密度、特定分类群的分类分布以及细菌浮游群落的八个主要成员中的六个成员在整个区域内保持一致。来自七个独立的 16S rRNA 基因克隆文库（共 1223 个序列）和荧光原位杂交的结果表明，微生物组合在斯科舍弧形列岛之间主要是同质的，其中α变形菌、γ变形菌和噬纤维菌-黄杆菌-拟杆菌群是主要的细菌群体。在所产生的 1223 个可用序列中，有 1087 个（89%）与海洋微生物具有≥97%的相似性，有 331 个（27%）与以前在永久性寒冷的北极和南极海洋环境中检测到的已发表序列相匹配。总之，结果表明，优势细菌浮游生物群体在不同地点之间保持一致，但在稀有生物多样性方面可能存在显著差异。
