Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA.
Vet Pathol. 2012 Sep;49(5):834-8. doi: 10.1177/0300985811432345. Epub 2012 Jan 24.
This study evaluates the immunoreactivity of 12 sex cord-stromal tumors of nonhuman primates (11 granulosa cell tumors and 1 luteoma). The markers selected are used in the characterization of gonadal tumors in dogs and other species, including cytokeratins AE1/AE3, GATA-4, inhibin-α, neuron-specific enolase, protein gene product 9.5, and vimentin. A normal nonhuman primate ovary was used as a control and to optimize immunolabeling. Staining was graded as follows: 0 (nonstaining), 1+ (< 10% positive cells), 2+ (10%-50% positive cells), and 3+ (> 50% positive cells). Calretinin, GATA-4, neuron-specific enolase, and vimentin were the most consistently expressed markers (12 of 12). Cytokeratins AE1/AE3 were also consistently expressed (11 of 12). Inhibin-α and protein gene product 9.5 were expressed in 8 and 10 sex cord-stromal tumors, respectively. Results indicate that immunoreactivity of nonhuman primate sex cord-stromal tumors is similar to that observed in other species and that calretinin, GATA-4, and neuron-specific enolase are the most consistently expressed markers in nonhuman primate sex cord-stromal tumors.
本研究评估了 11 例颗粒细胞瘤和 1 例黄体瘤共 12 例非人类灵长类动物的性索-间质肿瘤的免疫反应性。选择的标志物用于描述狗和其他物种的性腺肿瘤，包括细胞角蛋白 AE1/AE3、GATA-4、抑制素-α、神经元特异性烯醇化酶、蛋白基因产物 9.5 和波形蛋白。正常的非人类灵长类动物卵巢作为对照并优化免疫标记。染色分级如下：0（无染色）、1+（<10%阳性细胞）、2+（10%-50%阳性细胞）和 3+（>50%阳性细胞）。钙视网膜蛋白、GATA-4、神经元特异性烯醇化酶和波形蛋白是表达最一致的标志物（12 例中的 12 例）。细胞角蛋白 AE1/AE3 也表达一致（12 例中的 11 例）。抑制素-α和蛋白基因产物 9.5 在 8 例和 10 例性索-间质肿瘤中表达。结果表明，非人类灵长类动物性索-间质肿瘤的免疫反应性与其他物种观察到的相似，钙视网膜蛋白、GATA-4 和神经元特异性烯醇化酶是非人类灵长类动物性索-间质肿瘤中表达最一致的标志物。
