荧光寿命技术在医学应用中的应用。
Fluorescence lifetime techniques in medical applications.
机构信息
Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
出版信息
Ann Biomed Eng. 2012 Feb;40(2):304-31. doi: 10.1007/s10439-011-0495-y. Epub 2012 Jan 25.
Abstract
This article presents an overview of time-resolved (lifetime) fluorescence techniques used in biomedical diagnostics. In particular, we review the development of time-resolved fluorescence spectroscopy (TRFS) and fluorescence lifetime imaging (FLIM) instrumentation and associated methodologies which allow in vivo characterization and diagnosis of biological tissues. Emphasis is placed on the translational research potential of these techniques and on evaluating whether intrinsic fluorescence signals provide useful contrast for the diagnosis of human diseases including cancer (gastrointestinal tract, lung, head and neck, and brain), skin and eye diseases, and atherosclerotic cardiovascular disease.
摘要
本文概述了用于生物医学诊断的时间分辨（寿命）荧光技术。特别是，我们回顾了时间分辨荧光光谱（TRFS）和荧光寿命成像（FLIM）仪器的发展以及相关方法，这些方法允许对生物组织进行体内表征和诊断。重点介绍了这些技术的转化研究潜力，并评估了固有荧光信号是否为包括癌症（胃肠道，肺部，头颈部和脑部），皮肤和眼部疾病以及动脉粥样硬化性心血管疾病在内的人类疾病的诊断提供了有用的对比。
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