Noskov V N, Tarutina M G, Pavlov Iu I, Kulikov V N, Trofimova M V, Gorbacheva A V, Chernov Iu O, Sasnauskas K V, Neĭstat M A, Tolstorukov I I
Genetika. 1990 Jul;26(7):1161-8.
The collection of overlapping lys2 deletions (five in the chromosomal and seven in the plasmid LYS2 gene) is constructed in this work. The deletions overlap the whole coding region of the gene and provide the system for intragenic recombinational mapping of lys2 mutations in one of 14 controlled regions. A portion of these regions can be correlated with the regions on the physical map of LYS2. Mutations in two regions can be easily cloned. The system constructed gives the possibility for the study of intragenic and molecular specificity of mutagenesis.
在本研究中构建了重叠的lys2缺失文库（染色体上有5个，质粒LYS2基因中有7个）。这些缺失覆盖了该基因的整个编码区，并为14个控制区域之一中的lys2突变的基因内重组作图提供了系统。这些区域的一部分可与LYS2物理图谱上的区域相关联。两个区域中的突变能够很容易地被克隆。所构建的系统为研究诱变的基因内特异性和分子特异性提供了可能。
