Chernov Iu O, Gordenin D A
Genetika. 1987 Jan;23(1):30-40.
The lys2-32 mutant allele resulted from Ty1 element insertion was identified and cloned. The expression and reversions of lys2-32 localized in an autonomous plasmid were studied. The insertion was shown to inactivate LYS2 gene incompletely. Spontaneous reversions to complete or almost complete prototrophy were also obtained. About 50% of revertants retained the insertion. Others arise as a result of imprecise excision events leading to deletions of adjacent LYS2 sequences.
鉴定并克隆了由Ty1元件插入产生的lys2 - 32突变等位基因。研究了位于自主质粒中的lys2 - 32的表达和回复突变。结果表明，该插入使LYS2基因不完全失活。还获得了自发回复到完全或几乎完全原养型的突变体。约50%的回复突变体保留了插入。其他回复突变体是由于不精确切除事件导致相邻LYS2序列缺失而产生的。
