Ph1 基因座在小麦减数分裂前期和减数分裂过程中抑制 Cdk2 型激酶活性。
The Ph1 locus suppresses Cdk2-type activity during premeiosis and meiosis in wheat.
机构信息
John Ines Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
出版信息
Plant Cell. 2012 Jan;24(1):152-62. doi: 10.1105/tpc.111.094771. Epub 2012 Jan 24.
Abstract
Despite possessing multiple sets of related (homoeologous) chromosomes, hexaploid wheat (Triticum aestivum) restricts pairing to just true homologs at meiosis. Deletion of a single major locus, Pairing homoeologous1 (Ph1), allows pairing of homoeologs. How can the same chromosomes be processed as homologs instead of being treated as nonhomologs? Ph1 was recently defined to a cluster of defective cyclin-dependent kinase (Cdk)-like genes showing some similarity to mammalian Cdk2. We reasoned that the cluster might suppress Cdk2-type activity and therefore affect replication and histone H1 phosphorylation. Our study does indeed reveal such effects, suggesting that Cdk2-type phosphorylation has a major role in determining chromosome specificity during meiosis.
摘要
尽管六倍体小麦 (Triticum aestivum) 拥有多套相关的 (同源) 染色体，但它在减数分裂过程中仅将真正的同源染色体进行配对。缺失一个主要基因座 Pairing homoeologous1 (Ph1) 可以允许同源染色体配对。那么，同一对染色体如何被视为同源染色体而不是非同源染色体进行处理呢？Ph1 最近被定义为一组缺陷的细胞周期蛋白依赖性激酶（Cdk）样基因簇，与哺乳动物 Cdk2 有一定的相似性。我们推测该基因簇可能抑制 Cdk2 型活性，从而影响复制和组蛋白 H1 的磷酸化。我们的研究确实揭示了这样的影响，表明 Cdk2 型磷酸化在决定减数分裂过程中染色体特异性方面起着重要作用。
相似文献
1
The Ph1 locus suppresses Cdk2-type activity during premeiosis and meiosis in wheat.Ph1 基因座在小麦减数分裂前期和减数分裂过程中抑制 Cdk2 型激酶活性。
Plant Cell. 2012 Jan;24(1):152-62. doi: 10.1105/tpc.111.094771. Epub 2012 Jan 24.
2
Structural and functional relationship between the Ph1 locus protein 5B2 in wheat and CDK2 in mammals.小麦 Ph1 基因座蛋白 5B2 与哺乳动物 CDK2 的结构与功能关系。
Funct Integr Genomics. 2010 May;10(2):157-66. doi: 10.1007/s10142-010-0170-7. Epub 2010 Apr 27.
3
Detailed dissection of the chromosomal region containing the Ph1 locus in wheat Triticum aestivum: with deletion mutants and expression profiling.利用缺失突变体和表达谱对普通小麦中包含Ph1位点的染色体区域进行详细剖析。
Ann Bot. 2008 Apr;101(6):863-72. doi: 10.1093/aob/mcm252. Epub 2007 Oct 20.
4
The novel function of the Ph1 gene to differentiate homologs from homoeologs evolved in Triticum turgidum ssp. dicoccoides via a dramatic meiosis-specific increase in the expression of the 5B copy of the C-Ph1 gene.Ph1基因区分同源染色体和部分同源染色体的新功能是在野生二粒小麦中通过C-Ph1基因5B拷贝在减数分裂过程中特异性显著上调表达而进化产生的。
Chromosoma. 2019 Dec;128(4):561-570. doi: 10.1007/s00412-019-00724-6. Epub 2019 Sep 7.
5
Genetic effect of the Ph1 locus on transcriptome atlas of anther development-related genes, meiotic chromosome behavior and agronomic traits in bread wheat.Ph1位点对面包小麦花药发育相关基因转录组图谱、减数分裂染色体行为及农艺性状的遗传效应。
Plant J. 2025 May;122(3):e70203. doi: 10.1111/tpj.70203.
6
The molecular features of chromosome pairing at meiosis: the polyploid challenge using wheat as a reference.减数分裂中染色体配对的分子特征：以小麦为参照的多倍体挑战。
Funct Integr Genomics. 2010 May;10(2):147-56. doi: 10.1007/s10142-010-0171-6. Epub 2010 Apr 27.
7
TaASY1 promotes homologous chromosome interactions and is affected by deletion of Ph1.TaASY1促进同源染色体相互作用，并受Ph1缺失的影响。
Plant J. 2009 Feb;57(3):487-97. doi: 10.1111/j.1365-313X.2008.03701.x. Epub 2008 Sep 26.
8
Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat.Ph1作为多倍体小麦主要染色体配对位点的分子特征分析
Nature. 2006 Feb 9;439(7077):749-52. doi: 10.1038/nature04434.
9
Licensing MLH1 sites for crossover during meiosis.在减数分裂期间为交叉许可MLH1位点。
Nat Commun. 2014 Aug 6;5:4580. doi: 10.1038/ncomms5580.
10
Effect of the pairing gene Ph1 on centromere misdivision in common wheat.配对基因Ph1对普通小麦着丝粒错分裂的影响。
Genetics. 1998 Mar;148(3):1285-94. doi: 10.1093/genetics/148.3.1285.
引用本文的文献
1
Genomic Divergence Shaped the Genetic Regulation of Meiotic Homologous Recombination in Brassica Allopolyploids.基因组差异塑造了芸苔属异源多倍体减数分裂同源重组的遗传调控。
Mol Biol Evol. 2025 Apr 1;42(4). doi: 10.1093/molbev/msaf073.
2
Heterochromatin in plant meiosis.植物减数分裂中的异染色质。
Nucleus. 2024 Dec;15(1):2328719. doi: 10.1080/19491034.2024.2328719. Epub 2024 Mar 15.
3
Separating phases of allopolyploid evolution with resynthesized and natural .利用合成和自然异源多倍体来分离进化阶段。
Elife. 2024 Jan 8;12:RP88398. doi: 10.7554/eLife.88398.
4
The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency.具有不同重组频率的小麦位点的染色质决定因素和 Ph1 基因效应。
J Adv Res. 2023 Nov;53:75-85. doi: 10.1016/j.jare.2023.01.002. Epub 2023 Jan 9.
5
Bouquet Formation Failure in Meiosis of F Wheat-Rye Hybrids with Mitotic-Like Division.具有类有丝分裂分裂的F小麦-黑麦杂种减数分裂中花束形成失败
Plants (Basel). 2022 Jun 15;11(12):1582. doi: 10.3390/plants11121582.
6
Meiotic Chromosomal Abnormality Detected in a Heterozygote of .在……的杂合子中检测到的减数分裂染色体异常 。（原文中“of”后面内容缺失）
Front Plant Sci. 2022 May 3;13:895437. doi: 10.3389/fpls.2022.895437. eCollection 2022.
7
Meiosis in Polyploids and Implications for Genetic Mapping: A Review.多倍体减数分裂及其对遗传作图的影响：综述。
Genes (Basel). 2021 Sep 27;12(10):1517. doi: 10.3390/genes12101517.
8
Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.植物减数分裂中的泛素化：最新进展与高通量方法
Front Plant Sci. 2021 Apr 7;12:667314. doi: 10.3389/fpls.2021.667314. eCollection 2021.
9
Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.六倍体面包小麦减数分裂交叉的远端偏向反映了减数分裂程序的时空不对称性。
Front Plant Sci. 2021 Feb 12;12:631323. doi: 10.3389/fpls.2021.631323. eCollection 2021.
10
A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in .一种功能性激酶对于细胞周期蛋白依赖性激酶G1（CDKG1）在高温环境下维持生育能力是必需的。
Front Plant Sci. 2020 Nov 10;11:586870. doi: 10.3389/fpls.2020.586870. eCollection 2020.
本文引用的文献
1
The effect of Ph gene alleles on synaptonemal complex formation in Triticum aestivum × T. kotschyi hybrids.Ph 基因等位基因对普通小麦与节节麦杂种形成联会复合体的影响。
Theor Appl Genet. 1987 Aug;74(4):430-8. doi: 10.1007/BF00289817.
2
Inducing chromosome pairing through premature condensation: analysis of wheat interspecific hybrids.通过提前浓缩诱导染色体配对：小麦种间杂种的分析。
Funct Integr Genomics. 2010 Nov;10(4):603-8. doi: 10.1007/s10142-010-0185-0. Epub 2010 Jul 31.
3
Structural and functional relationship between the Ph1 locus protein 5B2 in wheat and CDK2 in mammals.小麦 Ph1 基因座蛋白 5B2 与哺乳动物 CDK2 的结构与功能关系。
Funct Integr Genomics. 2010 May;10(2):157-66. doi: 10.1007/s10142-010-0170-7. Epub 2010 Apr 27.
4
The molecular features of chromosome pairing at meiosis: the polyploid challenge using wheat as a reference.减数分裂中染色体配对的分子特征：以小麦为参照的多倍体挑战。
Funct Integr Genomics. 2010 May;10(2):147-56. doi: 10.1007/s10142-010-0171-6. Epub 2010 Apr 27.
5
Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels.复制工厂的激活可以通过调节 Cdk 水平与复制时间程序解耦。
J Cell Biol. 2010 Jan 25;188(2):209-21. doi: 10.1083/jcb.200911037. Epub 2010 Jan 18.
6
CDK2 is required for proper homologous pairing, recombination and sex-body formation during male mouse meiosis.在雄性小鼠减数分裂过程中，CDK2对于正确的同源配对、重组和性体形成是必需的。
J Cell Sci. 2009 Jun 15;122(Pt 12):2149-59. doi: 10.1242/jcs.046706.
7
Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation.黑麦Bs揭示了谷物基因组中的祖先序列，这些序列在配子体染色单体分离中可能发挥作用。
Mol Biol Evol. 2009 Aug;26(8):1683-97. doi: 10.1093/molbev/msp082. Epub 2009 Apr 24.
8
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification.MaxQuant可实现高肽段鉴定率、个体化的百万分之一级质量精度以及全蛋白质组范围的蛋白质定量。
Nat Biotechnol. 2008 Dec;26(12):1367-72. doi: 10.1038/nbt.1511. Epub 2008 Nov 30.
9
TaASY1 promotes homologous chromosome interactions and is affected by deletion of Ph1.TaASY1促进同源染色体相互作用，并受Ph1缺失的影响。
Plant J. 2009 Feb;57(3):487-97. doi: 10.1111/j.1365-313X.2008.03701.x. Epub 2008 Sep 26.
10
Effective chromosome pairing requires chromatin remodeling at the onset of meiosis.有效的染色体配对需要在减数分裂开始时进行染色质重塑。
Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6075-80. doi: 10.1073/pnas.0801521105. Epub 2008 Apr 15.
Zotero 插件