Department of Plant and Microbial Biology, Koshland Hall, University of California, Berkeley, CA, USA.
Plant Signal Behav. 2012 Jan;7(1):62-7. doi: 10.4161/psb.7.1.18636. Epub 2012 Jan 1.
Maize and Arabidopsis thaliana class 1 reversibly glycosylated polypeptides (C1RGPs) are plasmodesmata-associated proteins. Previously, over-expression of Arabidopsis C1RGP AtRGP2 in Nicotiana tabacum was shown to reduce intercellular transport of photoassimilate, resulting in stunted, chlorotic plants, and inhibition of local cell-to-cell spread of tobacco mosaic virus (TMV). Here, we used virus induced gene silencing to examine the effects of reduced levels of C1RGPs in Nicotiana benthamiana. Silenced plants show wild-type growth and development. Intercellular transport in silenced plants was probed using fluorescently labeled TMV and its movement protein, P30. P30 shows increased cell-to-cell movement and TMV exhibited accelerated systemic spread compared to control plants. These results support the hypothesis that C1RGPs act to regulate intercellular transport via plasmodesmata.
玉米和拟南芥 class 1 可逆糖基化多肽（C1RGPs）是胞间连丝相关蛋白。此前，在烟草中过表达拟南芥 C1RGP AtRGP2 被证明会减少光合同化物的细胞间运输，导致植株矮小、黄化，并抑制烟草花叶病毒（TMV）的局部细胞间扩散。在这里，我们使用病毒诱导的基因沉默来研究减少 Nicotiana benthamiana 中 C1RGPs 水平的影响。沉默的植物表现出野生型的生长和发育。用荧光标记的 TMV 及其运动蛋白 P30 探测沉默植物中的细胞间运输。与对照植物相比，P30 显示出增强的细胞间运动，TMV 表现出加速的系统传播。这些结果支持 C1RGPs 通过胞间连丝调节细胞间运输的假说。
