Biomedical Engineering, Graduate School of Medicine, Nara Medical University, Nara 634-8521, Japan.
J Biomater Appl. 2013 Mar;27(7):801-10. doi: 10.1177/0885328211426491. Epub 2012 Jan 24.
Surgical sealants form gel when applied to tissues. Currently, fibrin sealant has been successfully used in many surgical fields, but it has several disadvantages, including possible virus transmission, low adhesive strength, and high cost. In this study, gelatin and glutaraldehyde (GA) solutions were chosen to demonstrate the effectiveness of cross-linked gelatin gel as sealant and barrier, both of which have long been used in medical applications. It was found that the gelatin gel prepared from 26 wt% gelatin and 1 wt% GA solutions exhibited bonding strength almost three times higher than that of fibrin glue. The bonding strength increased with the increasing gelatin and GA concentrations. When a needle hole on PTFE vascular grafts was sealed with the gelatin gel, the water-resistant pressure significantly increased upon rubbing and was twice higher than that of fibrin glue. The cytotoxicity of gelatin gel was found to be much lower than that of albumin glue prepared at the same composition as commercially available BioGlue®. The gelatin gel was found to be also effective as barrier to prevent adhesion in a rat cecum abrasion model.
外科用黏合剂涂抹在组织上会形成凝胶。目前，纤维蛋白黏合剂已成功应用于许多外科领域，但它有几个缺点，包括可能传播病毒、黏附强度低和成本高。在这项研究中，选择了明胶和戊二醛（GA）溶液来证明交联明胶凝胶作为黏合剂和屏障的有效性，这两种物质在医学应用中已经使用了很长时间。结果发现，由 26wt%明胶和 1wt%GA 溶液制备的明胶凝胶的黏合强度几乎是纤维蛋白胶的三倍。随着明胶和 GA 浓度的增加，黏合强度也随之增加。当用明胶凝胶密封聚四氟乙烯血管移植物上的针孔时，耐水压力在摩擦时显著增加，是纤维蛋白胶的两倍。明胶凝胶的细胞毒性明显低于以与市售 BioGlue®相同的组成制备的白蛋白胶。明胶凝胶在大鼠盲肠擦伤模型中作为防止粘连的屏障也是有效的。
