Department of Chemistry, Texas A&M University, College Station, Texas 77842, United States.
ACS Nano. 2012 Feb 28;6(2):1503-12. doi: 10.1021/nn204431m. Epub 2012 Jan 25.
The strategy of decorating antibiofouling hyperbranched fluoropolymer-poly(ethylene glycol) (HBFP-PEG) networks with a settlement sensory deterrent, noradrenaline (NA), and the results of biofouling assays are presented. This example of a dual-mode surface, which combines both passive and active modes of antibiofouling, works in synergy to improve the overall antibiofouling efficiency against barnacle cyprids. The HBFP-PEG polymer surface, prior to modification with NA, was analyzed by atomic force microscopy, and a significant distribution of topographical features was observed, with a nanoscopic roughness measurement of 110 ± 8 nm. NA attachment to the surface was probed by secondary ion mass spectrometry to quantify the extent of polymer chain-end substitution with NA, where a 3- to 4-fold increase in intensity for a fragment ion associated with NA was observed and 39% of the available sites for attachment were substituted. Cytoskeletal assays confirmed the activity of tethered NA on adhering oyster hemocytes. Settlement assays showed deterrence toward barnacle cyprid settlement, while not compromising the passive biofouling resistance of the surface. This robust strategy demonstrates a methodology for the incorporation of actively antibiofouling moieties onto a passively antibiofouling network.
本文介绍了一种通过在具有抗生物污染的超支化氟聚合物-聚乙二醇（HBFP-PEG）网络上装饰具有沉降感觉威慑作用的去甲肾上腺素（NA）的策略，以及生物污染测定的结果。这种双模式表面的例子结合了被动和主动的抗生物污染模式，协同工作以提高对藤壶幼体的整体抗生物污染效率。在用 NA 修饰之前，通过原子力显微镜对 HBFP-PEG 聚合物表面进行了分析，观察到明显的形貌特征分布，纳米级粗糙度测量为 110±8nm。通过二次离子质谱探测 NA 与表面的附着，以定量聚合物链末端与 NA 的取代程度，观察到与 NA 相关的片段离子强度增加了 3-4 倍，并且有 39%的可用附着位点被取代。细胞骨架测定证实了附着在牡蛎血细胞上的 NA 的活性。沉降测定表明对藤壶幼体附着有威慑作用，同时不影响表面的被动抗生物污染阻力。这种稳健的策略展示了将主动抗生物污染部分结合到被动抗生物污染网络中的方法。
