Food Characterization and Quality Department, Instituto de la Grasa (CSIC), Seville, Spain.
J Agric Food Chem. 2012 Feb 22;60(7):1645-51. doi: 10.1021/jf2045312. Epub 2012 Feb 9.
Triacylglycerols of subcutaneous fat of Iberian pigs reared on two different feeding systems, extensive and intensive, have been determined by gas chromatography with a flame ionization detector. Analyses were performed on a column coated with a bonded stationary phase (50% phenyl-50% methylpolysiloxane) with hydrogen as the carrier gas. Lipids were extracted by melting the subcutaneous fat in a microwave oven and then filtering and dissolving in hexane. A total amount of 1995 samples from several campaigns were considered. Palmitoyl-stearyl-oleoyl glycerol and palmitoyl-dioleoyl glycerol were the most abundant triacylglycerols found in the samples. A study on the discriminating power of the triacylglycerols to differentiate samples according to the pig feeding system was performed. By using the triacylglycerols as chemical descriptors, principal component analysis, linear discriminant analysis, and soft independent modeling of class analogy were applied. Dioleoyl-linoleoyl glycerol and oleoyl-dilinoleoyl glycerol were the most discriminating variables. Variable-variable plots of these two glycerols allow separation of the samples according to their content.
用气相色谱法（配有火焰离子化检测器）测定了在两种不同饲养系统（粗放型和集约型）下饲养的伊比利亚猪皮下脂肪的三酰基甘油。分析是在涂有键合固定相（50%苯基-50%甲基聚硅氧烷）的柱子上进行的，载气为氢气。将皮下脂肪在微波炉中融化，然后过滤并溶解在己烷中，提取脂质。对多次实验中的总共 1995 个样本进行了分析。在所研究的样本中，最丰富的三酰基甘油是棕榈酰硬脂酰油酰甘油和棕榈酰二油酰甘油。研究了三酰基甘油对根据猪饲养系统区分样本的区分能力。通过将三酰基甘油用作化学描述符，应用了主成分分析、线性判别分析和类间独立软模式。二油酰亚油酸甘油和油酰亚油酰二甘油是最具判别力的变量。这两种甘油的变量-变量图允许根据它们的含量对样品进行分离。
