Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
Clin Oral Implants Res. 2013 May;24(5):531-5. doi: 10.1111/j.1600-0501.2011.02416.x. Epub 2012 Jan 26.
A limited number of experimental animal studies and in vitro studies have confirmed that nicotine impairs bone healing, diminishes osteoblast function, causes autogenous bone graft morbidity, and decreases graft biomechanical properties. The aim of this study was the histomrphometric assessment of the effect of nicotine on guided bone augmentation in a rat model.
Twenty-four male Wistar rats were randomly divided into a nicotine group and a control group. All animals received either nicotine (3 mg/kg) or saline 4 weeks before the surgical procedure and continued to receive nicotine or saline from surgery until death at 12 weeks. Two plastic caps were placed in the exposed calvaria of rats. Images of bone augmentation within the plastic caps were then taken using microfocus computed tomography (micro-CT). Histological sections were cut along the same plane as that used for micro-CT images.
Bone augmentation beyond the skeletal envelope occurred in both the nicotine and control groups. However, the nicotine group showed significantly smaller increases in bone volume and bone height than the controls.
Nicotine jeopardized, but did not prevent, the process of guided bone augmentation in a rat model.
少数实验动物研究和体外研究证实，尼古丁会损害骨愈合，减弱成骨细胞功能，导致自体骨移植物发病率增加，并降低移植物生物力学性能。本研究的目的是评估尼古丁对大鼠模型引导骨再生的影响。
24 只雄性 Wistar 大鼠随机分为尼古丁组和对照组。所有动物在手术前 4 周分别给予尼古丁（3mg/kg）或生理盐水，并且从手术开始至 12 周死亡期间继续给予尼古丁或生理盐水。将两个塑料帽放置在暴露的大鼠颅骨上。然后使用微焦点计算机断层扫描（micro-CT）拍摄塑料帽内骨再生的图像。沿着与 micro-CT 图像相同的平面切割组织学切片。
在尼古丁组和对照组中，均发生了超出骨骼范围的骨再生。然而，与对照组相比，尼古丁组的骨体积和骨高度增加明显较小。
尼古丁虽然没有完全阻止，但对大鼠模型的引导骨再生过程造成了危害。
