Karlsruhe Institute of Technology, Campus North, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Nanotoxicology. 2013 May;7(3):259-73. doi: 10.3109/17435390.2011.652206. Epub 2012 Jan 26.
In cell culture studies, foetal calf serum (FCS) comprising numerous different proteins is added, which might coat the surface of engineered nanomaterials (ENMs) and thus could profoundly alter their biological activities. In this study, a panel of industrially most relevant metal oxide nanoparticles (NPs) was screened for toxic effects in A549 lung epithelial cells and RAW264.7 macrophages in the presence and absence of FCS. In medium without FCS amorphous SiO2-NPs were the most cytotoxic NPs and induced a significant pro-inflammatory response in both cell types. An increased anti-oxidative response after exposure to SiO2-NPs was, however, only observed in RAW264.7 macrophages. Furthermore, pre-coating of SiO2-NPs with FCS proteins or simply bovine serum albumin abrogated responses in A549 lung epithelial cells. Thus, the protein corona bound to the surface of SiO2-NPs suppresses their biological effects, an issue which needs to be more carefully considered for in vitro-in vivo extrapolations.
在细胞培养研究中，添加了包含许多不同蛋白质的胎牛血清（FCS），这些蛋白质可能会覆盖工程纳米材料（ENMs）的表面，从而极大地改变它们的生物活性。在这项研究中，筛选了一组工业上最相关的金属氧化物纳米颗粒（NPs），以研究它们在存在和不存在 FCS 的情况下对 A549 肺上皮细胞和 RAW264.7 巨噬细胞的毒性作用。在不含 FCS 的培养基中，无定形 SiO2-NPs 是最具细胞毒性的 NPs，并在两种细胞类型中均诱导出明显的促炎反应。然而，仅在 RAW264.7 巨噬细胞中观察到暴露于 SiO2-NPs 后抗氧化反应增加。此外，用 FCS 蛋白或牛血清白蛋白预先包被 SiO2-NPs 可抑制 A549 肺上皮细胞的反应。因此，与 SiO2-NPs 表面结合的蛋白质冠抑制了它们的生物学效应，这是在体外-体内外推中需要更仔细考虑的问题。
