Déciga-Alcaraz Alejandro, Medina-Reyes Estefany I, Delgado-Buenrostro Norma L, Rodríguez-Ibarra Carolina, Ganem-Rondero Adriana, Vázquez-Zapién Gustavo J, Mata-Miranda Mónica M, Limón-Pacheco Jorge H, García-Cuéllar Claudia M, Sánchez-Pérez Yesennia, Chirino Yolanda I
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México. Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla de Baz, CP 54090, Estado de Mexico, Mexico.
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de Mexico, CDMX, 04510, Programa de becas posdoctorales en la UNAM, DGAPA, Mexico.
Toxicology. 2020 Sep;442:152545. doi: 10.1016/j.tox.2020.152545. Epub 2020 Aug 2.
The Organisation for Economic Co-operation and Development has listed thirteen engineered nanomaterials (ENM) in order to investigate their toxicity on human health. Silicon dioxide (SiO) and titanium dioxide (TiO) are included on that list and we added indium tin oxide (ITO) nanoparticles (NPs) to our study, which is not listed on OECD suggested ENM to be investigated, however ITO NPs has a high potential of industrial production. We evaluate the physicochemical properties of SiO NPs (10-20 nm), TiO nanofibers (NFs; 3 μm length) and ITO NPs (<50 nm) and the impact of protein-corona formation on cell internalization. Then, we evaluated the toxicity of uncoated ENM on human lung epithelial cells exposed to 10 and 50 μg/cm for 24 h. TiO NFs showed the highest capability to adsorb proteins onto the particle surface followed by SiO NPs and ITO NPs after acellular incubation with fetal bovine serum. The protein adsorption had no impact on Alizarin Red S conjugation, intrinsic properties for reactive oxygen (ROS) formation or cell uptake for all types of ENM. Moreover, TiO NFs induced highest cell alterations in human lung epithelial cells exposed to 10 and 50 μg/cm while ITO NPs induced moderated cytotoxicity and SiO NPs caused even lower cytotoxicity under the same conditions. DNA, proteins and lipids were mainly affected by TiO NFs followed by SiO NPs with toxic effects in protein and lipids while limited variations were detected after exposure to ITO NPs on spectra analyzed by Fourier Transform Infrared Spectroscopy.
经济合作与发展组织列出了13种工程纳米材料（ENM），以研究它们对人类健康的毒性。二氧化硅（SiO）和二氧化钛（TiO）在该列表中，我们将氧化铟锡（ITO）纳米颗粒（NPs）纳入我们的研究，尽管ITO NPs在工业生产中有很大潜力，但它未被列入经合组织建议研究的ENM列表中。我们评估了SiO NPs（10 - 20纳米）、TiO纳米纤维（NFs；长度为3微米）和ITO NPs（<50纳米）的物理化学性质以及蛋白质冠形成对细胞内化的影响。然后，我们评估了未包被的ENM对暴露于10和50微克/平方厘米24小时的人肺上皮细胞的毒性。与胎牛血清进行无细胞孵育后，TiO NFs在颗粒表面吸附蛋白质的能力最强，其次是SiO NPs和ITO NPs。蛋白质吸附对所有类型的ENM的茜素红S结合、活性氧（ROS）形成的内在特性或细胞摄取均无影响。此外，在暴露于10和50微克/平方厘米的人肺上皮细胞中，TiO NFs引起的细胞变化最大，而在相同条件下，ITO NPs引起中度细胞毒性，SiO NPs引起的细胞毒性更低。DNA、蛋白质和脂质主要受到TiO NFs的影响，其次是SiO NPs，对蛋白质和脂质有毒性作用，而在傅里叶变换红外光谱分析的光谱中，暴露于ITO NPs后检测到的变化有限。
