Department of Chemical and Environmental Engineering, University of the Basque Country, Plaza Europa 1, 20018 Donostia - San Sebastián, Spain.
Bioresour Technol. 2012 Mar;108:155-61. doi: 10.1016/j.biortech.2012.01.010. Epub 2012 Jan 10.
Ultrasonic treatments (0, 15, 30, 60 and 120 min) were applied to black liquor resulting from organosolv fractionation of olive tree pruning residues (ethanol/water 60/40 v/v, 180 °C, 60 min) in order to determine their effect on black liquor components. HPLC analyses of ultrasound-treated liquid fractions demonstrated that ultrasonic irradiation promoted up to 20% degradation of monosaccharides for 15 min of sonication and an increase of monomeric sugars from 3% to 16% due lignin-carbohydrate complex rupture. The quality and purity of the lignin precipitated from sonicated liquors by adding acidified water were assessed. Attenuated-total reflectance infrared spectroscopy (ATR-IR), gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR) confirmed that main lignin structure did not change due sonication, and thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and chemical composition and antioxidant behavior showed purification of lignin samples. These results established sonication as a suitable intensification technology in biorefinery processes.
超声处理（0、15、30、60 和 120 分钟）应用于由橄榄树修剪残渣的有机溶剂分级（乙醇/水 60/40v/v、180°C、60 分钟）得到的黑液中，以确定其对黑液成分的影响。超声处理的液体馏分的 HPLC 分析表明，超声辐射促进了 15 分钟的单糖降解，高达 20%，并由于木质素-碳水化合物复合物的破裂，单体糖从 3%增加到 16%。通过添加酸化水从超声处理的液体中沉淀的木质素的质量和纯度进行了评估。衰减全反射红外光谱（ATR-IR）、凝胶渗透色谱（GPC）和核磁共振（NMR）证实，由于超声处理，木质素的主要结构没有改变，而热重分析（TGA）、差示扫描量热法（DSC）以及化学成分和抗氧化行为表明木质素样品得到了纯化。这些结果确立了超声处理作为生物精炼过程中一种合适的强化技术。
