Department of Biology, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy.
Aquat Toxicol. 2012 Apr;110-111:107-13. doi: 10.1016/j.aquatox.2011.12.017. Epub 2011 Dec 31.
Surfactants are used for several purposes and recently they have attracted the attention for their ability to modify the behavior of other preexistent or co-disposed contaminants, although their use or discharge in wastewaters can represent a real or potential risk for the environment. Lemna minor L. and Azolla filiculoides Lam. are floating aquatic macrophytes, very effective in accumulating several pollutants including sodium dodecyl sulphate (SDS). In this work we evaluated the effects of SDS on these species by determining the stress ethylene production via laser-based trace gas detection, and the activities of enzymes involved in stress response, such as guaiacol peroxidase (G-POD), phenylalanine ammonia-lyase (PAL) and polyphenol-oxidase (PPO). Phenolics content was also determined. The macrophytes were treated with different concentrations of SDS for one week. SDS affected duckweed enzymatic activities and phenol content. While in the fern phenolics amount, PAL, G-POD and PPO activities were not affected by SDS except for 100 ppm SDS, the only concentration that was taken up and not completely degraded. Stress ethylene production was induced only in the fern treated with 50 and 100 ppm SDS.
表面活性剂具有多种用途，最近它们因其能够改变其他预先存在或共同处理的污染物的行为而引起了人们的关注，尽管它们在废水中的使用或排放可能对环境构成真正的或潜在的风险。浮萍和满江红是漂浮的水生植物，对包括十二烷基硫酸钠（SDS）在内的多种污染物的积累非常有效。在这项工作中，我们通过基于激光的痕量气体检测来确定胁迫乙烯的产生，以及参与胁迫反应的酶的活性，如愈创木酚过氧化物酶（G-POD）、苯丙氨酸解氨酶（PAL）和多酚氧化酶（PPO），来评估 SDS 对这些物种的影响。还测定了总酚含量。将大型植物用不同浓度的 SDS 处理一周。SDS 影响浮萍的酶活性和酚含量。而在蕨类植物中，除了 100ppm SDS 外，SDS 对酚类物质、PAL、G-POD 和 PPO 的活性没有影响，这是唯一被吸收但未完全降解的浓度。只有在 50ppm 和 100ppm SDS 处理的蕨类植物中才诱导产生胁迫乙烯。
