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植物中乙烯的检测方法。

Current methods for detecting ethylene in plants.

机构信息

Department of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, NL 6525 AJ Nijmegen, The Netherlands.

出版信息

Ann Bot. 2013 Mar;111(3):347-60. doi: 10.1093/aob/mcs259. Epub 2012 Dec 12.

Abstract

BACKGROUND

In view of ethylene's critical developmental and physiological roles the gaseous hormone remains an active research topic for plant biologists. Progress has been made to understand the ethylene biosynthesis pathway and the mechanisms of perception and action. Still numerous questions need to be answered and findings to be validated. Monitoring gas production will very often complete the picture of any ethylene research topic. Therefore the search for suitable ethylene measuring methods for various plant samples either in the field, greenhouses, laboratories or storage facilities is strongly motivated.

SCOPE

This review presents an update of the current methods for ethylene monitoring in plants. It focuses on the three most-used methods - gas chromatography detection, electrochemical sensing and optical detection - and compares them in terms of sensitivity, selectivity, time response and price. Guidelines are provided for proper selection and application of the described sensor methodologies and some specific applications are illustrated of laser-based detector for monitoring ethylene given off by Arabidopsis thaliana upon various nutritional treatments.

CONCLUSIONS

Each method has its advantages and limitations. The choice for the suitable ethylene sensor needs careful consideration and is driven by the requirements for a specific application.

摘要

背景

鉴于乙烯在植物发育和生理过程中的关键作用,这种气态激素仍然是植物生物学家的一个活跃研究课题。人们已经在理解乙烯生物合成途径以及感知和作用机制方面取得了进展。但仍有许多问题需要回答,许多发现需要验证。监测气体的产生通常可以完整呈现任何乙烯研究课题的情况。因此,强烈需要寻找适合各种植物样本的乙烯测量方法,无论是在田间、温室、实验室还是储存设施中。

范围

本文综述了目前植物中乙烯监测的方法。它重点介绍了三种最常用的方法——气相色谱检测、电化学传感和光学检测,并比较了它们在灵敏度、选择性、时间响应和价格方面的性能。为正确选择和应用描述的传感器方法提供了指导,并举例说明了基于激光的检测器在监测拟南芥受到各种营养处理时释放的乙烯方面的具体应用。

结论

每种方法都有其优点和局限性。对于合适的乙烯传感器的选择需要仔细考虑,并由特定应用的要求驱动。

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