Mimitsuka Takashi, Na Kyungsu, Morita Ken, Sawai Hideki, Minegishi Shinichi, Henmi Masahiro, Yamada Katsushige, Shimizu Sakayu, Yonehara Tetsu
New Frontiers Research Laboratories, Toray Industries, Inc., Kamakura, Kanagawa, Japan.
Biosci Biotechnol Biochem. 2012;76(1):67-72. doi: 10.1271/bbb.110499.
Continuous fermentation by retaining cells with a membrane-integrated fermentation reactor (MFR) system was found to reduce the amount of supplied sub-raw material. If the amount of sub-raw material can be reduced, continuous fermentation with the MFR system should become a more attractive process for industrialization, due to decreased material costs and loads during the refinement process. Our findings indicate that the production rate decreased when the amount of the sub-raw material was reduced in batch fermentation, but did not decrease during continuous fermentation with Sporolactobacillus laevolacticus. Moreover, continuous fermentation with a reduced amount of sub-raw material resulted in a productivity of 11.2 g/L/h over 800 h. In addition, the index of industrial process applicability used in the MFR system increased by 6.3-fold as compared with the conventional membrane-based fermentation reactor previously reported, suggesting a potential for the industrialization of this D-lactic acid continuous fermentation process.
通过膜集成发酵反应器（MFR）系统保留细胞进行连续发酵，可减少副原料的供应量。如果能够减少副原料的量，由于精炼过程中材料成本和负荷的降低，使用MFR系统进行连续发酵对于工业化来说将成为一个更具吸引力的工艺。我们的研究结果表明，在分批发酵中减少副原料量时生产率会下降，但在用左旋乳酸芽孢杆菌进行连续发酵时生产率并未下降。此外，使用减少量的副原料进行连续发酵在800小时内的生产率达到11.2 g/L/h。此外，与先前报道的传统膜基发酵反应器相比，MFR系统中使用的工业过程适用性指数提高了6.3倍，这表明该D-乳酸连续发酵工艺具有工业化潜力。
