Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Appl Microbiol Biotechnol. 2011 Feb;89(4):1009-17. doi: 10.1007/s00253-010-2904-9. Epub 2010 Oct 7.
Highly efficient D-lactate production by Sporolactobacillus sp. strain CASD was demonstrated in this study. Peanut meal was found to be a better nutrient than yeast extract, soybean meal, soybean peptone, corn steep, liquor beef extract, and ammonium sulfate in the production of D-lactate. To improve the utilization of peanut meal, the material was enzymatically hydrolyzed and simultaneously utilized as the nitrogen source in D-lactate fermentation. Very high D-lactate production (207 g/L) was obtained using 40 g/L of peanut meal in 30-L fed-batch fermentation, with the average productivity of 3.8 g/(L·h) and optical purity of 99.3%. The production of such a high concentration of optically pure D-lactate by strain CASD, with the simultaneous enzymatic hydrolysis of peanut meal and fermentation, represents a new cost-efficient and integrated method for D-lactate production using agricultural by-products.
本研究证明了 Sporolactobacillus sp. 菌株 CASD 能够高效生产 D-乳酸。与酵母提取物、豆粕、大豆蛋白胨、玉米浆、牛肉膏和硫酸铵相比，花生粕是生产 D-乳酸的更好的营养物质。为了提高花生粕的利用率，将其进行酶解，并同时用作 D-乳酸发酵中的氮源。在 30-L 分批补料发酵中使用 40 g/L 的花生粕，可获得非常高的 D-乳酸产量（207 g/L），平均产率为 3.8 g/（L·h），光学纯度为 99.3%。通过同时进行花生粕的酶解和发酵，菌株 CASD 能够生产如此高浓度的光学纯 D-乳酸，这代表了一种使用农业副产物生产 D-乳酸的新型、经济高效且集成的方法。
