用单克隆抗体对人心肌组织和分离的大鼠心肌细胞中的地高辛进行免疫荧光检测。
Immunofluorescence demonstration of digoxin in human myocardial tissue and isolated rat cardiac myocytes with monoclonal antibodies.
作者信息
Sótonyi P, Somogyi E, Rajs J, Sundberg M, Andersson B
机构信息
Department of Forensic Medicine of Semmelweis University of Medicine, Budapest, Hungary.
出版信息
Forensic Sci Int. 1990 Sep;47(2):157-63. doi: 10.1016/0379-0738(90)90209-h.
The aim of the present investigation was to identify the morphological correlates of digoxin binding sites in human heart muscle tissue and isolated viable rat heart myocytes. Cardiac glycoside linked to myocardial cells was demonstrated by monoclonal digoxin specific antibody and by FITC-conjugated anti-mouse immunoglobin serum. This versatile immunofluorescence method can be used in diagnostic and experimental studies of cardiac glycoside binding.
本研究的目的是确定地高辛结合位点在人心脏肌肉组织和分离的存活大鼠心肌细胞中的形态学关联。通过单克隆地高辛特异性抗体和异硫氰酸荧光素（FITC）偶联的抗小鼠免疫球蛋白血清来证实与心肌细胞相连的强心苷。这种通用的免疫荧光方法可用于强心苷结合的诊断和实验研究。
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