在培养的大鼠心肌细胞测试系统中，地高辛单克隆抗体的功能性拮抗作用。
Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes.
作者信息
Wallukat G, Simon H U, Müller W D, Wolf I
机构信息
Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany.
出版信息
Mol Cell Biochem. 1996 Jul-Aug;160-161:117-20. doi: 10.1007/BF00240040.
A monoclonal antibody with a high affinity for digitoxin (KA = 0.50 nM) and digoxin (KA = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication.
通过体细胞融合制备了一种对洋地黄毒苷（KA = 0.50 nM）和地高辛（KA = 0.55 nM）具有高亲和力的单克隆抗体。这种名为2A3(47)的抗体与其他糖苷几乎没有交叉反应。在培养的大鼠心肌细胞中，2A3(47)拮抗洋地黄毒苷所产生的正性变时作用，但不改变哇巴因的正性变时作用。我们的结果表明，这种单克隆抗体可能被证明可用于治疗地高辛和洋地黄毒苷中毒。
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