Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China.
Analyst. 2012 Mar 7;137(5):1199-204. doi: 10.1039/c2an15756j. Epub 2012 Jan 26.
In this article, the charge detection quadrupole mass spectrometry (CD-ITMS) driven by rectangular and triangular waveforms (rect-CD-ITMS and tri-CD-ITMS) was developed for the characterization of microparticles. Since the frequency scan of rectangular and triangular waveform could be realized easier than that of sinusoidal waveform, this research intends to provide simpler operation modes for CD-ITMS. In order to demonstrate the feasibility of rect-CD-ITMS and tri-CD-ITMS, the discharge onset voltage, ejection point of analyzed particles, and the achieved mass resolution were analyzed and compared with the case in conventional sinusoidal CD-ITMS (sin-CD-ITMS). The results indicated that the rect-CD-ITMS and tri-CD-ITMS can work well for the mass measurement of microparticles by using frequency scan. Identical mass resolutions were achieved under the same root mean square (RMS) voltage of different waveforms. The mass resolution was further improved by increasing the applied voltage and signal-to-noise ratio (S/N) of charge detector. Moreover, the rect-CD-ITMS and tri-CD-ITMS were applied to characterize red blood cells (RBCs). According to the obtained mean masses and mass distributions, normal and anemic RBCs were distinguished successfully.
本文采用矩形波和三角波（rect-CD-ITMS 和 tri-CD-ITMS）驱动的电荷检测四极杆质谱（CD-ITMS）对微粒进行了表征。由于矩形波和三角波的频率扫描比正弦波更容易实现，因此本研究旨在为 CD-ITMS 提供更简单的操作模式。为了验证 rect-CD-ITMS 和 tri-CD-ITMS 的可行性，分析并比较了放电起始电压、分析颗粒的喷射点以及实现的质量分辨率与传统正弦波 CD-ITMS（sin-CD-ITMS）的情况。结果表明，rect-CD-ITMS 和 tri-CD-ITMS 可以通过频率扫描很好地用于微粒的质量测量。在不同波形相同均方根（RMS）电压下实现了相同的质量分辨率。通过增加施加电压和电荷检测器的信噪比（S/N）进一步提高了质量分辨率。此外，rect-CD-ITMS 和 tri-CD-ITMS 被应用于表征红细胞（RBC）。根据获得的平均质量和质量分布，成功地区分了正常和贫血的 RBC。
