Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China.
Analyst. 2011 Apr 7;136(7):1305-9. doi: 10.1039/c0an00911c. Epub 2011 Feb 9.
A miniature cylindrical ion trap mass spectrometer (CIT-MS) equipped with an inexpensive mechanical pump was constructed, and used to measure the masses of cells and microparticles generated by laser induced acoustic desorption ionization. Compared with a previous lab scale quadrupole ion trap mass spectrometer (QIT-MS), the novel miniature CIT-MS had smaller volume (the radius r(0)=5 mm), simpler ion trap fabrication and overall instrument construction, required a lower trapping voltage, and reduced the weight, power and cost of the instrument. The CIT-MS was calibrated using standard polystyrene beads of 2.982 µm diameter. The CIT-MS was used to measure the total dry weight of human red blood cells (RBCs) from a healthy female adult (2.12×10(13) Da) and a patient with anemia (1.35×10(13) Da). The coefficient of variance (CV) for the healthy individual was 21% and that for the patient was 30.4%. The CIT-MS was also applied to guinea pig RBCs and the total dry weight was determined as 1.34×10(13) Da with a CV of 37.9%. These measurements are consistent with previous QIT-MS measurements. The new miniaturized instrument has potential for applications in field-portable, biological and aerosol analysis.
构建了一种配备廉价机械泵的微型圆柱离子阱质谱仪（CIT-MS），并将其用于测量激光诱导声解吸电离产生的细胞和微粒的质量。与以前的实验室规模四极离子阱质谱仪（QIT-MS）相比，新型微型 CIT-MS 具有更小的体积（半径 r(0)=5 毫米）、更简单的离子阱制造和整体仪器结构、更低的捕获电压，并且减少了仪器的重量、功耗和成本。CIT-MS 使用直径为 2.982 µm 的标准聚苯乙烯珠进行校准。CIT-MS 用于测量健康成年女性（2.12×10(13) Da）和贫血患者（1.35×10(13) Da）的人红细胞（RBC）的总干重。健康个体的变异系数（CV）为 21%，患者的 CV 为 30.4%。CIT-MS 还应用于豚鼠 RBC，并确定总干重为 1.34×10(13) Da，CV 为 37.9%。这些测量结果与之前的 QIT-MS 测量结果一致。新的小型化仪器具有在现场便携式、生物和气溶胶分析中应用的潜力。
