裂谷热病毒 RNA 基因组片段的编码区和非编码区在病毒 RNA 包装中的作用。
Roles of the coding and noncoding regions of rift valley Fever virus RNA genome segments in viral RNA packaging.
机构信息
Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, USA.
出版信息
J Virol. 2012 Apr;86(7):4034-9. doi: 10.1128/JVI.06700-11. Epub 2012 Jan 25.
Abstract
We characterized the RNA elements involved in the packaging of Rift Valley fever virus RNA genome segments, L, M, and S. The 5'-terminal 25 nucleotides of each RNA segment were equally competent for RNA packaging and carried an RNA packaging signal, which overlapped with the RNA replication signal. Only the deletion mutants of L RNA, but not full-length L RNA, were efficiently packaged, implying the possible requirement of RNA compaction for L RNA packaging.
摘要
我们对裂谷热病毒 RNA 基因组片段 L、M 和 S 的 RNA 元件进行了特征分析。每个 RNA 片段的 5' 端 25 个核苷酸都具有同等的 RNA 包装能力，并携带 RNA 包装信号，该信号与 RNA 复制信号重叠。只有 L RNA 的缺失突变体，而不是全长 L RNA，能够有效地被包装，这表明 L RNA 包装可能需要 RNA 紧缩。
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