McGrady A
J Cell Physiol. 1979 May;99(2):223-32. doi: 10.1002/jcp.1040990209.
The morphological aspects of spermatogenesis are well described in many mammalian species, but functional changes are not completely understood. Electrophysiological parameters were investigated in primary spermatocytes and early and late spermatids isolated from the seminiferous tubules of the mouse. Substantial changes were not detected in membrane potential between different developmental stages. Membrane potential was dependent on both potassium and sodium ion concentration gradients, but not on chloride gradients. The ratio of the permeabilities PNa/Pk varied according to the extracellular concentrations of sodium and potassium. Ouabain, a specific inhibitor of Na+, K+-activated ATPase, produced a maximal reduction in membrane potential of 20%. Comparisons were drawn between differentiating germ cells and previously determined properties of mature spermatozoa.
许多哺乳动物物种中精子发生的形态学方面已有详细描述，但功能变化尚未完全了解。对从小鼠曲细精管分离出的初级精母细胞、早期和晚期精子细胞的电生理参数进行了研究。不同发育阶段之间未检测到膜电位的显著变化。膜电位取决于钾离子和钠离子浓度梯度，但不取决于氯离子梯度。钠钾通透性比值PNa/Pk根据细胞外钠和钾的浓度而变化。哇巴因是Na+、K+激活的ATP酶的特异性抑制剂，可使膜电位最大降低20%。对分化中的生殖细胞与先前确定的成熟精子特性进行了比较。
