Redmann K, Walliser S
Arch Geschwulstforsch. 1981;51(1):96-102.
The inhibition of (Na+/K+) activated membrane ATPase by cardiac glycosides is electrophysiologically detectable in terms of a reduction of the transmembrane potential (TMP) of cells. Two hours after incubation in medium with 10(-4) mole/l ouabain added, the TMP of cells were measured by means of glass microelectrodes. Compared with untreated controls, HeLa and FL-cells, human embryo cells, skin fibroblasts, leukocytes, and exudate macrophages without exception showed a TMP reduction typical of the particular cell kind. Primary cultures of human embryo cells and skin fibroblasts revealed the highest sensitivity (approx. 60% TMP reduction), while lesser and relatively low sensitivities were observed for permanent cells (approx. 30% TMP reduction) and white blood cells (approx. 20% TMP reduction), respectively. Unlike the above, the ovarian tumor cells showed inter-individually varied reactions within the range of TMP changes from - 55% to + 33%. The majority of malignant tumours, in particular, exhibited only a weak reaction in the ouabain test or no reaction at all. On the other hand, ovarian cysts or other clinically benign tumors showed the normal ouabain effect. The experimental results obtained for the ovarian tumors are believed to demonstrate a defect of one or several transport enzymes, which effect is associated with the malignancy of cells and may be used as a functional in vitro marker for malignancy in the field of cell physiology.
强心苷对（钠+/钾+）激活的膜ATP酶的抑制作用，在电生理方面可通过细胞跨膜电位（TMP）的降低检测到。在添加了10^(-4)摩尔/升哇巴因的培养基中孵育两小时后，用玻璃微电极测量细胞的TMP。与未处理的对照相比，HeLa细胞、FL细胞、人胚胎细胞、皮肤成纤维细胞、白细胞和渗出液巨噬细胞无一例外都表现出特定细胞类型典型的TMP降低。人胚胎细胞和皮肤成纤维细胞的原代培养显示出最高的敏感性（TMP降低约60%），而永久性细胞（TMP降低约30%）和白细胞（TMP降低约20%）分别表现出较低和相对较低的敏感性。与上述情况不同，卵巢肿瘤细胞在TMP变化范围从-55%到+33%之间表现出个体间的差异反应。特别是大多数恶性肿瘤在哇巴因试验中仅表现出微弱反应或根本没有反应。另一方面，卵巢囊肿或其他临床良性肿瘤表现出正常的哇巴因效应。卵巢肿瘤的实验结果被认为证明了一种或几种转运酶的缺陷，这种效应与细胞的恶性程度相关，并且可以作为细胞生理学领域中恶性肿瘤的功能性体外标志物。
