Hamilton P W, Allen D C, Watt P C
Department of Pathology, Royal Victoria Hospital/Queen's University of Belfast, N. Ireland.
Histopathology. 1990 Jul;17(1):59-68. doi: 10.1111/j.1365-2559.1990.tb00664.x.
Morphometry and stereology were used to assess the cytological and architectural characteristics of regenerative and dysplastic colorectal mucosal abnormalities in ulcerative colitis. Reproducibility of measurements was assessed and found to be good, confirming the objective and reproducible nature of morphometric analysis. Discriminant analysis between the morphometric features of regenerative mucosal change and low-grade dysplasia revealed the significant cytological and architectural variables from which a cytological and architectural score were derived. The architectural score was capable of classifying all of the cases into their correct diagnostic category, although a combination of the two scores provided better separation of cases. Probability density analysis was carried out so that probabilities of group membership could be allocated to cases, given their cytological and architectural scores. Discriminant analysis was also applied to low- and high-grade dysplasia. Important cytological and architectural variables were identified and used separately to derive scoring systems. In combination, the dual scoring of lesions was capable of separating low- from high-grade dysplasia, although overlap was still evident. Again, probability density analysis allowed the allocation of cases into one or other category, although a closer examination showed that such a rule could not successfully classify a new set of low- and high-grade dysplasia cases. Quantitative histological analysis of mucosal abnormalities is shown to be of use in the objective diagnosis of reactive and dysplastic change in patients with ulcerative colitis. The use of simple scoring systems and probability based allocation of cases promotes the future role of morphometric techniques in the diagnostic laboratory.
采用形态测量学和体视学方法评估溃疡性结肠炎中再生性和发育异常性大肠黏膜异常的细胞学和结构特征。评估了测量的可重复性，发现其良好，证实了形态测量分析的客观性和可重复性。对再生性黏膜改变和低度发育异常的形态学特征进行判别分析，揭示了从中得出细胞学和结构评分的重要细胞学和结构变量。结构评分能够将所有病例正确分类到其诊断类别中，尽管两种评分的组合能更好地区分病例。进行概率密度分析，以便根据病例的细胞学和结构评分将其归入相应组别的概率。判别分析也应用于低度和高度发育异常。确定了重要的细胞学和结构变量，并分别用于得出评分系统。虽然重叠仍然明显，但病变的双重评分能够区分低度和高度发育异常。同样，概率密度分析允许将病例归入一个或另一个类别，尽管仔细检查表明这样的规则不能成功地对一组新的低度和高度发育异常病例进行分类。黏膜异常的定量组织学分析在溃疡性结肠炎患者反应性和发育异常性改变的客观诊断中显示出有用性。简单评分系统的使用和基于概率的病例分类促进了形态测量技术在诊断实验室中的未来作用。
