Allen D C, Hamilton P W, Watt P C, Biggart J D
Histopathology Laboratory, Belfast City Hospital, Northern Ireland.
Histopathology. 1988 Jun;12(6):611-21. doi: 10.1111/j.1365-2559.1988.tb01985.x.
Semi-automatic image analysis was used to assess the architectural features of normal colorectal mucosa and ulcerative colitis with and without dysplasia. Eight measured and derived morphometric variables were compared with the histological grading. The main data-set variation was due to: (1) the area of mucosa and epithelium per unit length of muscularis mucosae; (2) mean mucosal and epithelial height; and (3) the percentage epithelium and number of crypts per unit length of muscularis mucosae. Discriminant analysis using the variables mean epithelial height and mean lamina propria area per unit length of muscularis mucosae separated normals (n = 10) from high-grade dysplasia (8). The classification rule allocated low-grade dysplasia (8) to the high-grade category and 60% of regeneration cases (10) to the normal mucosa group. Scatter plots of the two discriminating variables separated normal and regenerative mucosa from dysplasia. Histological review of overlapping cases allowed redesignation of a high-grade dysplasia lesion as low grade. Architectural morphometry may be of use in assessing premalignant mucosal changes in ulcerative colitis as a guide to patient surveillance and therapy.
采用半自动图像分析评估正常结直肠黏膜以及伴有和不伴有发育异常的溃疡性结肠炎的结构特征。将八个测量和推导得出的形态计量学变量与组织学分级进行比较。主要数据集的差异归因于：（1）每单位黏膜肌层长度的黏膜和上皮面积；（2）黏膜和上皮的平均高度；（3）每单位黏膜肌层长度的上皮百分比和隐窝数量。使用变量黏膜肌层每单位长度的上皮平均高度和固有层平均面积进行判别分析，可将正常组（n = 10）与高级别发育异常组（8例）区分开来。分类规则将低级别发育异常（8例）归为高级别类别，60%的再生病例（10例）归为正常黏膜组。两个判别变量的散点图将正常和再生黏膜与发育异常区分开来。对重叠病例进行组织学复查后，可将一个高级别发育异常病变重新指定为低级别。结构形态计量学可用于评估溃疡性结肠炎的癌前黏膜变化，作为患者监测和治疗的指导。
