Verity Software House, Topsham, Maine, USA.
Cytometry A. 2012 Jun;81(6):523-6. doi: 10.1002/cyto.a.22018. Epub 2012 Jan 25.
The Flow Cytometry Standard (FCS) format was developed back in 1984. Since then, FCS became the standard file format supported by all flow cytometry software and hardware vendors. Over the years, updates were incorporated to adapt to technological advancements in both flow cytometry and computing technologies. However, flexibility in how data may be stored in FCS has led to implementation difficulties for instrument vendors and third party software developers. In this technical note, we are providing implementation guidance and examples related to FCS 3.1, the latest version of the standard. By publishing this text, we intend to prevent potential compatibility issues that could be faced when implementing the FCS spillover and preferred display keywords that have arisen during discussions among some implementers.
流式细胞术标准 (FCS) 格式早在 1984 年就已开发。自那时以来，FCS 成为所有流式细胞术软件和硬件供应商支持的标准文件格式。多年来，为了适应流式细胞术和计算技术的技术进步，对其进行了更新。然而，FCS 中数据存储方式的灵活性给仪器供应商和第三方软件开发人员带来了实施困难。在本技术说明中，我们提供了与标准的最新版本 FCS 3.1 相关的实现指导和示例。通过发布此文本，我们旨在防止在实现 FCS 溢出和首选显示关键字时可能出现的潜在兼容性问题，这些关键字是在一些实现者的讨论中产生的。
