星形孢菌素在盘基网柄菌中诱导了不依赖聚（ADP - 核糖）聚合酶的细胞死亡。

Staurosporine induced poly (ADP-ribose) polymerase independent cell death in Dictyostelium discoideum.

作者信息

Mir Hina, Rajawat Jyotika, Begum Rasheedunnisa

机构信息

Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, India.

出版信息

Indian J Exp Biol. 2012 Jan;50(1):80-6.

PMID:22279946

Abstract

In the present study D. discoideum has been used as a model organism to understand the role of poly (ADP-ribose) polymerase (PARP) in caspase independent paraptotic cell death pathways. D. discoideum lacks caspases and Bcl-2 family proteins; nevertheless it has 9 potential genes for PARP. PARP has been known to get activated in various cell death associated diseases. In this study kinetics of cell death induced by staurosporine (STS), a bacterial alkaloid, was established to unravel the role of PARP. It was found that STS induced cell death in D. discoideum did not involve PARP activation, however it involved cathepsin D. Results indicated that an alternative mechanism may be existing in D. discoideum that lacks Bcl-2 family proteins for STS induced cell death that evades Bax involvement.

摘要

在本研究中，盘基网柄菌被用作模式生物，以了解聚（ADP - 核糖）聚合酶（PARP）在不依赖半胱天冬酶的副凋亡细胞死亡途径中的作用。盘基网柄菌缺乏半胱天冬酶和Bcl - 2家族蛋白；然而，它有9个潜在的PARP基因。已知PARP在各种与细胞死亡相关的疾病中被激活。在本研究中，建立了由细菌生物碱星形孢菌素（STS）诱导的细胞死亡动力学，以阐明PARP的作用。结果发现，STS诱导盘基网柄菌细胞死亡不涉及PARP激活，但涉及组织蛋白酶D。结果表明，在缺乏Bcl - 2家族蛋白的盘基网柄菌中，可能存在一种替代机制来介导STS诱导的细胞死亡，从而避免Bax的参与。