Vordermeier H M, Pope M, Kotlarski I
Department of Microbiology and Immunology, University of Adelaide, Australia.
Immunol Cell Biol. 1990 Jun;68 ( Pt 3):161-72. doi: 10.1038/icb.1990.23.
A comparison of the ability of normal peritoneal cells (PC) and those harvested from mice 1-3 days after intraperitoneal immunization with live Salmonella enteritidis 11RX (11RX) to present antigen to 11RX-primed T cells was made using formalin-killed 11RX and a soluble 11RX antigen extract as antigens. Unfractionated PC and the adherent and non-adherent PC populations were analysed separately and the effects of the lysosomal function-impairing drug chloroquine and the fixative paraformaldehyde, used before or after antigen-pulsing, were also determined. The results presented indicate that immunization with live 11RX did not induce any detectable modulation of APC function which could account for the ability of live 11RX to induce cell-mediated immune responses involving Lyt 2+ T cells.
使用福尔马林灭活的肠炎沙门氏菌11RX（11RX）和可溶性11RX抗原提取物作为抗原，比较正常腹膜细胞（PC）以及在腹腔注射活肠炎沙门氏菌11RX（11RX）后1 - 3天从小鼠体内获取的腹膜细胞向经11RX致敏的T细胞呈递抗原的能力。分别分析了未分离的PC以及黏附性和非黏附性PC群体，并确定了在抗原脉冲之前或之后使用的溶酶体功能损害药物氯喹和固定剂多聚甲醛的作用。给出的结果表明，用活11RX免疫不会诱导任何可检测到的抗原呈递细胞（APC）功能调节，这可以解释活11RX诱导涉及Lyt 2 + T细胞的细胞介导免疫反应的能力。
