Regional Blood Center of Ribeirão Preto-FUNDHERP, Faculty of Medicine in Ribeirão Preto-FMRP, University of São Paulo-USP, 2051 Tenente Catão Roxo Str., Ribeirão Preto, São Paulo, Brazil.
Can J Microbiol. 2012 Feb;58(2):200-5. doi: 10.1139/w11-119. Epub 2012 Jan 26.
Human parvovirus B19 (B19V) infection can be a life-threatening condition among patients with hereditary (chronic) hemolytic anemias. Our objective was to characterize the infection molecularly among patients with sickle cell disease and thalassemia. Forty-seven patients (37 with sickle cell disease, and 10 with β-thalassemia major) as well as 47 healthy blood donors were examined for B19V infection by anti-B19V IgG enzyme immunoassay, quantitative PCR, which detects all B19V genotypes, and DNA sequencing. B19V viremia was documented in nine patients (19.1%) as two displayed acute infection and the rest had a low titre viremia (mean 3.4 × 10(4) copies/mL). All donors were negative for B19V DNA. Anti-B19V IgG was detected in 55.3% of the patients and 57.4% among the donors. Based on partial NS1 fragments, all patient isolates were classified as genotype 1 and subgenotype 1A. The evolutionary events of the examined partial NS1 gene sequence were associated with a lack of positive selection. The quantification of all B19V genotypes by a single hydrolytic probe is a technically useful method, but it is difficult to establish relationships between B19V sequence characteristics and infection outcome.
人细小病毒 B19（B19V）感染可在遗传性（慢性）溶血性贫血患者中导致危及生命的状况。我们的目的是对镰状细胞病和地中海贫血患者的感染进行分子特征分析。通过抗 B19V IgG 酶联免疫吸附试验、定量 PCR（可检测所有 B19V 基因型）和 DNA 测序，对 47 名患者（37 名镰状细胞病患者和 10 名β地中海贫血重型患者）和 47 名健康献血者进行 B19V 感染检查。9 名患者（19.1%）出现 B19V 病毒血症，其中 2 名显示急性感染，其余患者病毒血症滴度较低（平均 3.4×10(4)拷贝/mL）。所有献血者的 B19V DNA 均为阴性。55.3%的患者和 57.4%的献血者检测到抗 B19V IgG。根据部分 NS1 片段，所有患者分离株均分类为基因型 1 和亚基因型 1A。所检测的部分 NS1 基因序列的进化事件与缺乏正选择有关。通过单个水解探针对所有 B19V 基因型进行定量是一种技术上有用的方法，但很难建立 B19V 序列特征与感染结果之间的关系。
