羊驼和公羊附属性腺、睾丸及精浆中的糖胺聚糖

Glycosaminoglycans in the accessory sex glands, testes and seminal plasma of alpaca and ram.

作者信息

Kershaw-Young Claire M, Evans G, Maxwell W M C

机构信息

Faculty of Veterinary Science, The University of Sydney, Camperdown, Sydney, NSW 2006, Australia.

出版信息

Reprod Fertil Dev. 2012;24(2):362-9. doi: 10.1071/RD11152.

DOI:10.1071/RD11152

PMID:22281083

Abstract

The viscous nature of alpaca semen limits its use in cryopreservation and other assisted reproductive technologies. The cause and source of this viscosity is unknown although it has been postulated, but never proven, that glycosaminoglycans (GAGs) secreted by the bulbourethral gland are responsible. The present study investigated the concentration and composition of GAGs in alpaca seminal plasma, testes, bulbourethral gland and prostate gland and compared them to those in the ram to determine the relationship between seminal plasma GAGs and viscosity and to identify the source of seminal plasma GAGs. Alpaca seminal plasma contained more GAGs than ram (P<0.001) and the predominant GAG, keratan sulfate, was correlated with viscosity (P=0.05, R(2)=0.2635). The alpaca bulbourethral gland contained most GAGs compared with prostate or testis (P<0.001). In the ram, the prostate contained most GAGs. These findings suggest that GAGs, particularly keratan sulfate, may be the cause of seminal plasma viscosity in alpacas, and that the seminal plasma GAGs originate from the bulbourethral gland.

摘要

羊驼精液的粘性限制了其在冷冻保存及其他辅助生殖技术中的应用。尽管有人推测但从未得到证实，认为尿道球腺分泌的糖胺聚糖（GAGs）是造成这种粘性的原因，而这种粘性的成因及来源尚不清楚。本研究调查了羊驼精浆、睾丸、尿道球腺和前列腺中GAGs的浓度及组成，并将其与公羊的进行比较，以确定精浆GAGs与粘性之间的关系，并找出精浆GAGs的来源。羊驼精浆中的GAGs含量高于公羊（P<0.001），且主要的GAG——硫酸角质素与粘性相关（P=0.05，R(2)=0.2635）。与前列腺或睾丸相比，羊驼尿道球腺含有的GAGs最多（P<0.001）。在公羊中，前列腺含有的GAGs最多。这些发现表明，GAGs，尤其是硫酸角质素，可能是羊驼精浆粘性的成因，且精浆GAGs来源于尿道球腺。