Faculty of Veterinary Science, The University of Sydney, Camperdown, Sydney, NSW, Australia.
Theriogenology. 2011 Oct 15;76(7):1197-206. doi: 10.1016/j.theriogenology.2011.05.016. Epub 2011 Aug 6.
In order to advance the development of assisted reproductive technologies in alpacas and other Camelids, the objective of this study was to explore the role of seminal plasma concentration on motility and functional integrity of alpaca sperm. Sixteen male alpacas > 3 y of age were used. In Experiment 1, epididymal sperm were incubated for 0 to 6 h in 0, 10, 25, 50, or 100% seminal plasma and motility was assessed. In Experiment 2, epididymal sperm were incubated in 0, 10, or 100% seminal plasma for 3 h and motility, acrosome integrity and DNA integrity were assessed. In Experiment 3, ejaculated sperm were incubated in 10, 25, 50, or 100% seminal plasma for 0 to 6 h and motility assessed. In Experiment 4, ejaculated sperm were incubated in 10 or 100% seminal plasma for 3 h and motility, acrosome integrity, DNA integrity, and viability were assessed. Epididymal and ejaculated sperm maintained motility longer when incubated in the presence of 10% seminal plasma compared to 0, 25, 50, or 100% seminal plasma (P < 0.001). The mean ± SEM percentage of epididymal sperm with intact acrosomes was less (P < 0.001) in samples incubated in 0% seminal plasma (39.4 ± 3.73) compared to 10% (75.3 ± 1.20) or 100% (77.4 ± 0.90) within 1 h after incubation. However, DNA integrity of ejaculated and epididymal sperm was not significantly affected by seminal plasma concentration. The mean viability of ejaculated sperm was reduced in the presence of 100 (12.7 ± 2.33) compared to 10% (36.2 ± 4.68) seminal plasma (P < 0.001) within 1 h of incubation. We concluded that alpaca semen should be diluted to a final concentration of 10% seminal plasma to prolong motility, preserve acrosome integrity, and maintain viability of sperm.
为了促进羊驼和其他骆驼科动物辅助生殖技术的发展，本研究旨在探讨精浆浓度对羊驼精子活力和功能完整性的作用。使用了 16 只年龄大于 3 岁的雄性羊驼。在实验 1 中，将附睾精子在 0%、10%、25%、50%或 100%精浆中孵育 0 至 6 小时，评估精子活力。在实验 2 中，将附睾精子在 0%、10%或 100%精浆中孵育 3 小时，评估精子活力、顶体完整性和 DNA 完整性。在实验 3 中，将射出的精子在 10%、25%、50%或 100%精浆中孵育 0 至 6 小时，评估精子活力。在实验 4 中，将射出的精子在 10%或 100%精浆中孵育 3 小时，评估精子活力、顶体完整性、DNA 完整性和活力。与 0%、25%、50%或 100%精浆相比，当在 10%精浆中孵育时，附睾精子的活力保持时间更长（P < 0.001）。在孵育 1 小时内，与 0%精浆（39.4 ± 3.73）相比，在 10%（75.3 ± 1.20）或 100%（77.4 ± 0.90）精浆中孵育的附睾精子完整顶体的比例显著降低（P < 0.001）。然而，精浆浓度对射出和附睾精子的 DNA 完整性没有显著影响。在孵育 1 小时内，与 10%精浆（36.2 ± 4.68）相比，100%精浆中射出精子的平均活力显著降低（12.7 ± 2.33）（P < 0.001）。我们得出结论，羊驼精液应稀释至终浓度为 10%的精浆，以延长精子活力、保持顶体完整性并维持精子活力。
