从地榆中提取的新型儿茶素 pilosanol N 抑制 LPS/IFN-γ 刺激的 RAW264.7 巨噬细胞产生一氧化氮。
Suppression of nitric oxide production on LPS/IFN-γ-stimulated RAW264.7 macrophages by a novel catechin, pilosanol N, from Agrimonia pilosa Ledeb.
机构信息
Department of Bioresource Technology, Okinawa National College of Technology, Nago City, Okinawa, Japan.
出版信息
Bioorg Med Chem Lett. 2012 Feb 15;22(4):1766-9. doi: 10.1016/j.bmcl.2011.12.086. Epub 2012 Jan 3.
A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by (1)H, (13)C NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-γ -induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-γ treatment cells were also suppressed in the presence of 1. In addition, compound 1 scavenged NO or nitrogen radicals generated from NOR3 (4-ethyl-2-hydroxyamino-5-nitro-3-hexenamide) as an NO donor. These results indicated that pilosanol N can decrease the level of NO through a mechanism that involved both a decrease in the NO production and NO scavenging.
从龙牙草（ Agrimonia pilosa Ledeb）中分离得到一种新的儿茶素化合物 pilosanol N（1），并通过（1）H、（13）C NMR 和 HRESI-MS 分析确定其结构。化合物 1 抑制 LPS/IFN-γ 诱导的 RAW264.7 巨噬细胞中一氧化氮（NO）的产生，并且在存在 1 的情况下，还抑制 LPS/IFN-γ 处理细胞中的 iNOS 基因表达及其蛋白产物。此外，化合物 1 清除了作为 NO 供体的 NOR3（4-乙基-2-羟基氨基-5-硝基-3-己烯酰胺）产生的 NO 或氮自由基。这些结果表明，pilosanol N 可以通过降低 NO 产生和清除 NO 的机制来降低 NO 水平。
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